A simple in situ synthesis of iron oxide magnetic nanoparticles embedded in thermosensitive polymer for DNA capture
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A simple in situ synthesis of iron oxide magnetic nanoparticles embedded in thermosensitive polymer for DNA capture Sadia Hossain1, Mahbubor Rahman1,2,a) , Yeasmin Nahar1, Abdur Rahman1, Mostafa Kaiyum Sharafat3, Motahar Hossain1, Bungo Ochiai4, Abdelhamid Elaissari5, Hasan Ahmad1 1 Research Laboratory of Polymer Colloids & Nanomaterials, Department of Chemistry, Faculty of Science, Rajshahi University, Rajshahi 6205, Bangladesh 2 Advance Materials Research Laboratory, Department of Materials Science and Engineering, Clemson University, Clemson, SC 29625-0971, USA 3 Department of Chemistry, Begum Rokeya University, Rangpur, Bangladesh 4 Department of Chemistry and Chemical Engineering, Graduate School of Science and Engineering, Yamagata University, Yonezawa, Japan 5 Laboratory of Automatic Control, Chemical and Pharmaceutical Engineering (LAGEP)-Lyon, University of Lyon 1, Villeurbanne Cedex 69622, France a) Address all correspondence to this author. e-mail: [email protected]
Received: 20 March 2020; accepted: 6 July 2020
In this study, we report a simple one-pot synthesis of iron oxide nanoparticles (IONPs) modified with thermoresponsive polymers potentially applicable for nucleic acid capture. Ferrous (Fe2+) and ferric (Fe3+) ions were coprecipitated to a dispersion of previously prepared poly(N-isopropylacrylamide-co-2-aminoethyl methacrylate) P(NIPAAm-co-AEM) for in situ synthesis of magnetite (Fe3O4) and concurrent surface modification of Fe3O4 with the polymer to obtain magnetic nanocomposites. Fourier-transform infrared (FTIR) spectroscopy analysis reveals the surface modification of Fe3O4 with P(NIPAAm-co-AEM) and P(NIPAAm) as functional and control polymers, respectively. Fe3O4@P(NIPAAm-co-AEM) and Fe3O4@P(NIPAAm) nanocomposites’ surfaces contain 7.5 and 2.3 wt% of immobilized polymers, respectively. Vibrating sample magnetometry (VSM) result indicates a high saturation of magnetization value, 75 emu/g, for Fe3O4@P(NIPAAm-co-AEM) nanocomposites. The hydrodynamic diameter of Fe3O4@P(NIPAAm-co-AEM) in water changes depending on pH and temperature. A study for deoxyribonucleic acid (DNA) capture ability of Fe3O4@P(NIPAAm-co-AEM) nanocomposites shows a maximum 18.5 mg/g of DNA can be adsorbed on Fe3O4@P(NIPAAm-co-AEM).
Introduction A wide variety of nanocarriers, such as liposomes, polymers, inorganic–organic hybrid nanoparticles, and metal–organic frameworks with diverse size, architectures, and surface properties, has been developed for drug delivery [1, 2]. The controlled drug delivery system based on magnetic nanoparticles and guided by an external magnetic field is a promising strategy to provide drug release at specific sites on demand to overcome the problems associated with conventional nanoparticle vehicles [3, 4]. Magnetic iron oxide nanoparticles (IONPs) have already emerged as promising candidates for drug delivery, magnetic resonance imaging, cell mechanics, hyperthermia, tumor progression, and separation of nucleic acids and cells due to their ultrafine sizes, biocompatibility, and
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