Acute Lung Injury in Response to Intratracheal Instillation of Lipopolysaccharide in an Animal Model of Emphysema Induce
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ORIGINAL ARTICLE
Acute Lung Injury in Response to Intratracheal Instillation of Lipopolysaccharide in an Animal Model of Emphysema Induced by Elastase Maria Aparecida Esteves Rabelo,1,2 Leda Marília Fonseca Lucinda,1,2,3 Maycon Moura Reboredo,1,3 Lídia Maria Carneiro da Fonseca,1,3 Fernando Fonseca Reis,1,3 Thaís Fernanda Fazza,1,3 Danielle Ribeiro Brega,1 Flavia de Paoli,2 Adenilson de Souza da Fonseca,4 and Bruno Valle Pinheiro1,3,5
Abstract—The response of lungs with emphysema to an acute lung injury (ALI) remains
unclear. This study compared the lung response to intratracheal instillation of lipopolysaccharide (LPS) in rats with and without emphysema. Twenty-four Wistar rats were randomized to four groups: control group (C-G), ALI group (ALI-G), emphysema group (E-G), emphysema and ALI group (E-ALI-G). Euthanasia and the following analysis were performed 24 h after ALI induction: lung histology, bronchoalveolar lavage (BAL), mRNA expression of inflammatory mediators, and blood gas measures. The histological analysis showed that animals of ALI-G (0.55 ± 0.15) and E-ALI-G (0.69 ± 0.08) had a higher ALI score compared to C-G (0.12 ± 0.04) and E-G (0.16 ± 0.04) (p < 0.05). The analysis of each component of the score demonstrated that ALI-G and E-ALI-G had greater alveolar and interstitial neutrophil infiltration, as well as greater amount of alveolar proteinaceous debris. Comparing the two groups that received LPS, there was a trend of higher ALI in the E-ALI-G, specially due to a higher neutrophil infiltration in the alveolar spaces and a higher septal thickening. Total cell count (E-G = 3.09 ± 0.83; ALI-G = 4.45 ± 1.9; E-ALI-G = 5.9 ± 2.1; C-G = 0.73 ± 0.37 × 105) and neutrophil count (E-G = 0.69 ± 0.35; ALI-G = 2.53 ± 1.09; E-ALI-G = 3.86 ± 1.4; CG = 0.09 ± 0.07 × 105) in the BAL were higher in the groups E-G, ALI-G, and E-ALI-G when compared to C-G (p < 0.05). The IL-6, TNF-α, and CXCL2 mRNA expressions were higher 1
Pulmonary Research Laboratory, Federal University of Juiz de Fora, Av. Eugênio do Nascimento - s/n°, Dom Bosco, Juiz de Fora, Minas Gerais 36038-330, Brazil 2 Department of Morphology, Institute of Biological Sciences, Federal University of Juiz de Fora, Rua José Lourenço Khelmer - s/n, Campus Universitário, São Pedro, Juiz de Fora, Minas Gerais 36036-900, Brazil 3 Center of Reproductive Biology, Federal University of Juiz de Fora, Rua José Lourenço Khelmer - s/n, Campus Universitário, São Pedro, Juiz de Fora, Minas Gerais 36036-900, Brazil 4 Department of Biophysics and Biometry, Institute of Biology Roberto Alcantara Gomes, Rio de Janeiro State University, Rua São Francisco Xavier, 524, Maracanã, Rio de Janeiro, 20550900, Brazil 5 To whom correspondence should be addressed at Pulmonary Research Laboratory, Federal University of Juiz de Fora, Av. Eugênio do Nascimento - s/n°, Dom Bosco, Juiz de Fora, Minas Gerais 36038-330, Brazil. E-mail: [email protected]
0360-3997/17/0000-0001/0 # 2017 Springer Science+Business Media, LLC
Rabelo, Lucinda, Reboredo, da Fonseca, Reis, Fazza, Brega,
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