Proteomic profiling of serum samples from chikungunya-infected patients provides insights into host response
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CLINICAL PROTEOMICS
RESEARCH
Open Access
Proteomic profiling of serum samples from chikungunya-infected patients provides insights into host response Vinuth N Puttamallesh1†, Sreelakshmi K Sreenivasamurthy1†, Pradeep Kumar Singh2, H C Harsha1, Anjali Ganjiwale3, Shobha Broor2, Akhilesh Pandey1,4,5,6,7*, Jayasuryan Narayana3* and T S Keshava Prasad1*
Abstract Background: Chikungunya is a highly debilitating febrile illness caused by Chikungunya virus, a single-stranded RNA virus, which is transmitted by Aedes aegypti or Aedes albopictus mosquito species. The pathogenesis and host responses in individuals infected with the chikungunya virus are not well understood at the molecular level. We carried out proteomic profiling of serum samples from chikungunya patients in order to identify molecules associated with the host response to infection by this virus. Results: Proteomic profiling of serum obtained from the infected individuals resulted in identification of 569 proteins. Of these, 63 proteins were found to be differentially expressed (≥ 2-fold) in patient as compared to control sera. These differentially expressed proteins were involved in various processes such as lipid metabolism, immune response, transport, signal transduction and apoptosis. Conclusions: This is the first report providing a global proteomic profile of serum samples from individuals infected with the chikungunya virus. Our data provide an insight into the proteins that are involved as host response factors during an infection. These proteins include clusterin, apolipoproteins and S100A family of proteins. Keywords: iTRAQ, Quantitative proteomics, Arthritis, Hyponatremia, Neuropathology
Background Chikungunya is an endemic disease in Africa and SouthEast Asia. Some isolated cases have also been reported in parts of Europe, Australia and America in the past decade [1-3]. Although the infection was considered to be arthritogenic, cases of meningoencephalitis and subsequent mortality have also been documented [4-6]. The persistent and painful arthralgia observed in this disease significantly reduces the ability to work among the affected.
* Correspondence: [email protected]; [email protected]; [email protected] † Equal contributors 1 Institute of Bioinformatics, International Technology Park, Bangalore 560 066, India 3 Microtest Innovations Pvt. Limited, International Technology Park, Bangalore 560 066, India Full list of author information is available at the end of the article
Chikungunya virus (CHIKV) is an arbovirus belonging to the family Togaviridae and genus Alphavirus. Its genome consists of 12 kb single-stranded RNA, which codes for 4 non-structural and 5 structural proteins. A mutation in the viral genome leading to a single amino acid change of alanine to valine at the position 226 (A226V) of the E1 glycoprotein [7], enhances its survival and transmission through the widely distributed vector- Aedes albopictus [8,9]. This has also been shown to enhance the dissemination of the virus into temperate regions, in addition t
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