An analysis of exo- polygalacturonase bioprocess in submerged and solid-state fermentation by Pleurotus ostreatus using
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Journal of Genetic Engineering and Biotechnology
RESEARCH
Open Access
An analysis of exo-polygalacturonase bioprocess in submerged and solid-state fermentation by Pleurotus ostreatus using pomelo peel powder as carbon source Kausik Majumder1,2*, Bubai Paul1 and Rakhi Sundas1
Abstract Background: As there has been an increasing trend in the effective utilization of plant and crop residues for microbial transformation into a desired product, an attempt was made to compare of exo-polygalacturonase production using logistic and Luedeking-Piret kinetic model by Pleurotus ostreatus in submerged (smf) or solid-state fermentation (ssf) using pomelo peel powder, an agro-forestry residue as carbon substrate. Results: Cultures grown in submerged fermentation produced a peak of exo-polygalacturonase activity as 6160 Ul-1 on the 4th day of culture as compared with 2410 Ul-1 on the 5th day of fermentation by solid-state fermentation. The enzyme yield coefficient (YE/X) is of higher value in smf vs. ssf system (YE/X = 1.05 × 103 vs. 0.622 × 103) indicating the more efficient product yield in smf as compared with ssf. The plots derived fromλ versusζ clearly demonstrate that the secondary product destruction is higher in smf than in ssf. Conclusion: P. ostreatus performs much better in submerged fermentation as compared with solid-state fermentation in respect to exo-polygalacturonase production although ssf technique produced a more thermostable exo-polygalacturonase in crude extract, which is highly desirable in various industrial applications. Keywords: Polygalacturonase, Pleurotus, Pomelo peel powder
Background Microbial pectinolytic enzymes of fungal origin have many industrial applications viz food processing, textile, etc. with tremendous potentials [1]. Filamentous fungi, e.g., Aspergillus niger are the most frequently used microorganism in the enzyme industry since they produce about 90% of enzyme extra-cellularly. Research reports are available on exo-polygalacturonase production by Aspergillus species using a wide range of substrates through either ssf or smf. Moreover, comparative * Correspondence: [email protected] 1 Post-Graduate Department of Botany, Darjeeling Government College, Government of West Bengal, Darjeeling, West Bengal 734101, India 2 Present address: Department of Botany, Bidhannagar College, Government of West Bengal, EB-2, Sector-1, Salt Lake City, Kolkata, West Bengal 700 064, India
assessment between these two techniques, i.e., smf and ssf on the production of polygalacturonase are also reported [2, 3] in which Aspergillus niger was used. There are very few works available about the production of polygalacturonase from the edible fungi, such as Lentinus edodes [4] or Pleurotus ostreatus [5]. These studies are confined either submerged or solid-state fermentation, although no research work has been on record about the comparative assessment on the kinetics of exo-polygalacturonase (exo-PG, EC 3.2.1.67) production by these two techniques. Moreover, enzymes obtained from the edible fungi
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