Analysis of Pharmaceuticals by Capillary Electrophoresis
During the 1980's the analysis of pharmaceuticals was dominated by the use of High Perfor mance Liquid Chromatography (HPLC). Other separative techniques such as Gas Chromato graphy and Thin Layer Chromatography offered alternatives but their quantitati
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CH ROMAIOGRAPH IA
CE Series
Edited by Kevin D. Altria, Glaxo Wellcome R&D, UK There are currently a number of general textbooks covering Capillary Electrophoresis where information on a range of applications and techniques can be found. Readers who are interested in a specific area of CE struggle to find truly comprehensive treatments of their areas of interest. The CHROMATOGRAPHIA CE series has been established to allow comprehensive books to be produced covering individual topics. The books are written by well known authors in their specialist application areas and cover CE topics such as DNA analysis, analysis of pharmaceuticals, chiral separations, MECC, carbohydrate analysis, biomedical applications and troubleshooting inCE .
• Volume 1: C. Heller (Ed.), Analysis of Nucleic Acids by Capillary Electrophoresis • Volume 2: K. D. Altria, Analysis of Pharmaceuticals by Capillary Electrophoresis
Kevin D. Altria
Analysis of Pharmaceuticals by Capillary Electrophoresis
II vleweg
All rights reserved © Friedr. Vieweg & Sohn Vedagsgesellschaft mbH, BraunschweiglWiesbaden, 1998
Softcover reprint of the hardcover 1st edition 1998
Vieweg is a subsidiary company of Bertelsmann Professional Information.
No part of this publication may be produced, stored in a retrieval system or transmitted, mechanical, photocopying or otherwise, without prior permission of the copyright holder.
http://www.vieweg.de Produced by Lengericher Handelsdruckerei, Lengerich
ISBN-13: 978-3-322-85013-3 DOl: 10.1007/978-3-322-85011-9
e-ISBN-13: 978-3-322-85011-9
v
Preface During the 1980's the analysis of pharmaceuticals was dominated by the use of High Performance Liquid Chromatography (HPLC). Other separative techniques such as Gas Chromatography and Thin Layer Chromatography offered alternatives but their quantitative capabilities and/or solute range could not approach that of HPLC. The majority of pharmaceuticals are ionic and it would be reasonable to assume that electrophoresis may be useful in the analysis of pharmaceuticals. However, the electrophoretic instruments available in the 1980's were labour intensive and employed post-separation detection procedures. During the late 1980's and early 1990's extensive research was conducted into the possibilities of conducting electrophoretic separations in capillaries. This approach allowed on-line detection and could be performed on fully automated equipment. This research led to the advent of modern day capillary electrophoresis (CE) instruments which offer similar performance and automation levels to that of HPLC. Research was also focused on developing applications for CE and particular attention was paid to applications within the pharmaceutical analysis area. These applications proved that CE could be applied to a wide range of drug types including water insoluble and neutral compounds. The ability to achieve efficient chiral separations of drugs also increased the popularity of the technique. CE with indirect UV detection has become established as a simple and effective altern
