Analysis of the Endophytic Bacteria Community Structure and Function of Panax notoginseng Based on High-Throughput Seque
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Analysis of the Endophytic Bacteria Community Structure and Function of Panax notoginseng Based on High‑Throughput Sequencing Chen Zhang1 · Xuan Ma2 · Runqi Zhu3 · Zhenzhen Liu1 · Mengmeng Gu1 · Jingyuan Zhang1 · Yu Li1 · Yanyan Xu1 · Daheng Zhu1 Received: 4 June 2019 / Accepted: 30 May 2020 © Springer Science+Business Media, LLC, part of Springer Nature 2020
Abstract Panax notoginseng has long been used as a Chinese herb with high medicinal value. The endophytic bacteria in this medicinal plant have multiple biological functions. High-throughput sequencing is a rapidly evolving technique that helps profile the endophytic bacterial community structure of medicinal plants. However, few studies on the endophytic bacteria in P. notoginseng, particularly in dry P. notoginseng roots as a raw medicinal material, have been conducted. In this study, fresh P. notoginseng and dry P. notoginseng were analysed using high-throughput sequencing on an Illumina MiSeq platform to explore the diversity and functions of the endophytic bacteria in different parts of P. notoginseng. The results showed that a total of 201 operational taxonomic units were obtained from fresh P. notoginseng and dry P. notoginseng. The dominant phyla in the fresh and dry P. notoginseng were Proteobacteria (85.9%) and Firmicutes (99.9%), respectively, whereas the dominant genera in these samples were Enterobacter (84.4%) and Bacillus (99.6%), respectively. Fresh P. notoginseng exhibited a higher degree of endophytic bacterial diversity than dry P. notoginseng, but functional prediction of metabolism indicated that the relative abundance of the metabolic function of terpenoids and polyketides synthesis in the dry sample was higher than that in the fresh sample. Our study indicates significant differences in the diversity and metabolic function of the endophytic bacteria between fresh and dry P. notoginseng, providing useful information for the exploitation and utilization of endophytic bacteria resources from P. notoginseng.
Introduction Panax notoginseng is a special kind of traditional Chinese herb with high medicinal value specifically grown in the Wenshan region of Yunnan Province [1], and a variety of bioactive components have been obtained from P. Electronic supplementary material The online version of this article (doi:https://doi.org/10.1007/s00284-020-02068-2) contains supplementary material, which is available to authorized users. * Daheng Zhu [email protected] 1
College of Life Science, Zhengzhou University, Henan 450000, China
2
Antu Biological Engineering Co., Ltd. Zhengzhou, Zhengzhou 450000, China
3
College of Pharmacy, Huazhong University of Science and Technology, Wuhan 430030, China
notoginseng [2], including polysaccharides, flavonoids and notoginsenoside [3]. Plant endophytes play a vital role in host growth, stress resistance and synthesis and transformation of secondary metabolites [4–6]. The interactions between endophytes and host plants may often lead to the synthesis of new kinds of compounds, which are usual
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