Analytical Biotechnology
Modern analytical biotechnology is focused on the use of a set of enabling platform technologies that provide contemporary, state-of-the-art tools for genomics, proteomics, metabolomics, drug discovery, screening, and analysis of natural product molecules
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Analytical Biotechnology Edited by Thomas G.M. Schalkhammer
Springer Basel AG
Editor Prof. Dr. Thomas G.M. Schalkhammer TUDELFT Kluyver Lab. for Biotechnology Julianalaan 67 2628 BC DELFT The Netherlands Library of Congress Cataloging-in-Publication Data Analytical biotechnology / edited by Thomas G.M. Schalkhammer. p. cm. - (Methods and tools in biosciences and medicine) Includes bibliographical references and index. 1. Analytical biotechnlogy. 2. Analytical biochemistry - Methodology. Thomas G.M. 1961-. II. Series.
r. Schalkhammer,
Deutsche Bibliothek Cataloging-in-Publication Data Analytical biotechnology / ed. by Thomas G. M. Schalkhammer. - Basel; Boston; Berlin: Birkhăuser, 2002 (Methods and tools in biosciences and medicine)
ISBN 978-3-7643-6589-9 ISBN 978-3-0348-8101-2 (eBook) DOI 10.1007/978-3-0348-8101-2 The publisher and editor can give no guarantee for the information on drug dosage and administration contained in this publication. The respective user must check its accuracy by consulting other sources of reference in each individual case. The use ofregistered names, trademarks etc. in this publication, even ifnot identified as such, does not imply that they are exempt from the relevant protective laws and regulations or free for general use. This work is subject to copyright. AlI rights are reserved, whether the whole or part of the material is concerned, specifically the rights of translation, reprinting, re-use of illustrations, recitation, broadcasting, reproduction on microfilms or in other ways, and storage in data banks. For any kind of use permission of the copyright owner must be obtained. © 2002 Springer Basel AG Originally published by Birkhăuser Verlag, Basel in 2002 Softcover reprint of the hardcover 1st edition 2002 Member of the BertelsmannSpringer Publishing Group Printed on acid-free paper produced from chlorine-free pulp. TCFoo Cover illustration: Color assay using assemblies of DNA-conjugated nano-clusters (see p. 264) 987654321
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v Contents Ust of Contributors......................................................................................
VII
Preface ..........................................................................................................
IX
1 Immobilized Biomolecules in Bioanalysis.......................................... Fritz Pittner
1
2 Fluorescence Techniques.................................................................... Christian Mayer and Thomas G. M. Schalkhammer
43
3 Immunoanalytical Methods................................................................. Franz Gabor, Oskar Hoffmann, Fritz Pittner and Michael Wirth
93
4 Immunological Strip Tests................................................................... 134 Ron Verheijen 5 Biosensors......................................... .......... ...................... .................... 167 Thomas G. M. Schalkhammer 6 Biocbips. ............................ .......................... .......................... ................ Norbert Stich
220
7 Nano-clusters
