Anthocyanins Profile by Q-TOF LC/MS in Myrtus communis Berries from Salento Area
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Anthocyanins Profile by Q-TOF LC/MS in Myrtus communis Berries from Salento Area Sonia Scorrano 1 & Maria Rosaria Lazzoi 1 Roberta Del Sole 1 & Giuseppe Vasapollo 1
&
Lucia Mergola 1 & Maria Pia Di Bello 1 &
Received: 2 November 2016 / Accepted: 10 January 2017 # Springer Science+Business Media New York 2017
Abstract In the present study, it was defined the complete anthocyanins profile in Myrtus communis berries, a perennial wild shrub typical of the Mediterranean area. To the best of our knowledge, the anthocyanin composition of myrtle berries in above area has never been described. So, because of beneficial effects of these compounds reported during the last decades in the literature, an anthocyanins extraction from berries of Salento area was performed. The pigments were extracted with 0.1% HCl in methanol and purified using a C-18 solidphase cartridge in about 24 h. High-performance liquid chromatography/electrospray ionization quadrupole time-offlight mass spectrometry (LC/ESI-Q-TOF) method was developed optimizing chromatographic parameters in order to obtain an unequivocal identification of anthocyanins. Comparing to the conventional technologies employed for anthocyanins identification (HPLC-DAD and triple quadrupole), the Q-TOF used in this work is a last generation mass spectrometry technique that demonstrated to be able to provide accurate data on anthocyanins identification. The results obtained showed that delphinidin 3-O-glucoside represented about 31.5%, petunidin 3-O-glucoside was about 25.8%, and malvidin 3-O-glucoside represented 24.3% of the total pigments. Other anthocyanins found in minor amounts were delphinidin-pentose (4%), delphinidin-pentose (3.8%), cyanidin 3-O-glucoside (6.3%), petunidin-pentose (0.7%), petunidin-pentose (1.6%), and peonidin 3-O-glucoside (2%). The characterization of the anthocyanins profile in Myrtus communis berries of Salento, characteristic shrubs of
* Maria Rosaria Lazzoi [email protected]
1
Department of Engineering for Innovation, University of Salento, via per Arnesano Km. 1, 73100 Lecce, Italy
Mediterranean region, make these fruits an excellent source of natural antioxidants compounds, which can be very useful in new biotechnological applications in food and pharmaceutical fields. Keywords Anthocyanins . Myrtus communis . Mediterranean scrub . LC/ESI-Q-TOF . Natural pigments
Introduction The development of new nutraceuticals and supplemented foods based on extracts or compounds obtained from different natural sources such as fruits or vegetables is taking much interest. Anthocyanins are a representative example of this trend as on onset nutraceutical industry is currently attracted in this class of compounds because of their beneficial effects reported in the literature. So, interests in these water-soluble pigments have been increased substantially during the last decade because of the increasing evidence demonstrating potential therapeutic effects to human health occasioned by their free-radical scavenging, antioxidant capacities, a
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