Anti-inflammation effects of the total saponin fraction from Dioscorea nipponica Makino on rats with gouty arthritis by
- PDF / 3,502,068 Bytes
- 13 Pages / 595.276 x 790.866 pts Page_size
- 96 Downloads / 172 Views
(2020) 20:261
BMC Complementary Medicine and Therapies
RESEARCH ARTICLE
Open Access
Anti-inflammation effects of the total saponin fraction from Dioscorea nipponica Makino on rats with gouty arthritis by influencing MAPK signalling pathway Qi Zhou1* , Hui Juan Sun2, Shu Min Liu3, Xi Hong Jiang2, Qiu Yue Wang2, Shuang Zhang2 and Dong Hua Yu1
Abstract Background: Dioscorea nipponica Makino is widely used in traditional Chinese medicine to treat gouty arthritis. Methods: Sixty male Wistar rats were divided into six groups: the normal group, model group, colchicine group (COL) and three total saponin groups (RDN) (high dose [160 mg/kg], middle dose [80 mg/kg] and low dose [40 mg/ kg]). HE staining was used to detect the histopathologic changes of the synovial tissue of joint. Immunohistochemical method was used to detect the protein expressions of P-38, p-P38, JNK, p-JNK, ERK1/2, pERK1/2, MEK1/2, p-MEK1/2, MKK4, p-MKK4, ICAM1, VCAM1, and PPARĪ³ in the synovial tissue of joint. Realtime PCR and WB methods were used to detect the mRNA and protein expressions of PPARĪ³ and AdipoR2 in the synovial tissue of joint. The contents of CXCL1 and ADP in the blood serum were measured by Elisa method. Results: Our study showed that RDN could improve the situation of the synovial tissue, reduce the protein expressions of MKK4, p-MEK1/2, p-JNK, p-ERK1/2, ICAM1. They could also decrease the content of CXCL1 and increase the content of ADP in the blood serum. Conclusion: RDN has good effect of anti-inflammation. This is in part realized by influencing MAPK signalling pathway. It provides a new visual angle to reveal the mechanism of RDN to treat GA. Keywords: Dioscorea nipponica Makino, Gouty arthritis (GA), Mitogen-activated protein kinase (MAPK), Peroxisome proliferators-activated receptors (PPARs)
Background Gouty arthritis (GA) has recently been occurring more frequently in China and worldwide [1]. It is common in men compared to women [2]. This disorder results from high serum uric acid concentrations; monosodium urate (MSU) crystals are subsequently formed and deposited in both joints and tissues. Symptoms occur during acute episodes, and extreme pain is noted in this period [3]. * Correspondence: [email protected] 1 Research Institute of Chinese Medicine, Heilongjiang University of Chinese Medicine, Harbin 150040, P.R. China Full list of author information is available at the end of the article
Comorbidities are common in gout patients, such as arrhythmias, obesity, diabetes mellitus, and chronic kidney disease [4, 5]. Current drug used to treat GA may have some side effects which could be seen obviously from our previous study, such as stomach injury by indomethacin. It is urgent to identify new drugs to treat GA since it was detrimental to the health of the patients and especially in the condition of comorbidities [6]. Mitogen-activated protein kinases (MAPKs) play an important role during the pathogenesis of GA [7]. MAPKs are classified into main three types: extracellular signal-regulated kinase-1/2 (ERK1/2), c-Jun N
Data Loading...
