The protective effects of Terminalia laxiflora extract on hepato-nephrotoxicity induced by fipronil in male rats
- PDF / 2,184,678 Bytes
- 9 Pages / 595.276 x 790.866 pts Page_size
- 19 Downloads / 245 Views
RESEARCH ARTICLE
The protective effects of Terminalia laxiflora extract on hepato-nephrotoxicity induced by fipronil in male rats A. A. Khalaf 1 & Marwa A. Ibrahim 2
&
Mona K. Galal 2 & Amr A. Abdallah 3 & Ragaa Mansour 4 & Mostafa M. Afify 5
Received: 25 December 2019 / Accepted: 6 July 2020 # Springer-Verlag GmbH Germany, part of Springer Nature 2020
Abstract The present study was led to investigate the defensive role of Terminalia laxiflora extract (TLE) on fipronil (FPN) induced hepatotoxicity and nephrotoxicity in male rats. Rats were administered with TLE (100 mg/kg) against the renal toxicity and hepatotoxicity induced by administration of FPN (10.5 mg/kg) for 30 days. At the end of the experimental period, the serum, liver, and kidneys were harvested and assessed for subsequent analysis. FPN administration to rats resulted in a significant elevation of serum transaminases, urea, and creatinine. Also, FPN-treated groups exhibited a marked reduction in total protein and albumin levels. Compared with the control group, the level of malondialdehyde (MDA) was elevated in groups treated with FPN, whereas superoxide dismutase (SOD), catalase (CAT) activities, and glutathione levels were distinctly reduced in this group. Significant increases in genomic DNA fragmentation and the expression level of the caspase-3 gene were also recorded. The biochemical result was supported by histopathological findings. Co-administration of TLE along with FPN significantly diminished the liver and kidney function tests decreased the level of lipid peroxidation, and enhanced all the antioxidant enzymes, while also diminishing the expression of caspase-3 and DNA laddering, indicating amelioration of DNA damage. These results indicate that TLE plays a vital role in diminishing FPN-induced hepatotoxicity and nephrotoxicity. Keywords Terminalia laxiflora . Fipronil . Hepatotoxicity . Nephrotoxicity . DNA damage . Caspase-3
Introduction Fipronil (FPN) is an exceedingly effective, broad-spectrum insecticide that is broadly used to control a wide scope of agricultural, public health, and veterinary pests. This insecticide belongs to a new class of phenylpyrazole pesticides that
have been developed to reduce environmental and mammalian damage (Wang et al. 2016). Their toxicity results in the outcome from the capacity to block gamma-aminobutyric acid-gated chloride channels of neurons in the central nervous system. Currently, exposure to phenylpyrazole pesticides is a worldwide general medical problem and many concerns have
Responsible Editor: Lotfi Aleya * Marwa A. Ibrahim [email protected]
1
Department of Forensic Medicine and Toxicology, Faculty of Veterinary Medicine, Cairo University, Giza, Egypt
2
Department of Biochemistry and Molecular Biology, Faculty of Veterinary Medicine, Cairo University, Giza 12211, Egypt
3
Mammalian Toxicology Department, Central Agricultural Pesticides Lab, CAPL., Agriculture Research Center, ARC., Dokki, Giza, Egypt
Ragaa Mansour [email protected]
4
National Research Center, Dokki,
