Antibacterial and antiparasitic activities analysis of a hepcidin-like antimicrobial peptide from Larimichthys crocea
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Antibacterial and antiparasitic activities analysis of a hepcidinlike antimicrobial peptide from Larimichthys crocea Libing Zheng1, 3, Yuan Li2, Jun Wang1, Ying Pan1, Jia Chen1, Weiqiang Zheng1, Longshan Lin2* 1 State Key Laboratory of Large Yellow Croaker Breeding, Ningde 352103, China 2 Third Institute of Oceanography, Ministry of Natural Resources, Xiamen 361005, China 3 National and Provincial Joint Laboratory of Exploration and Utilization of Marine Aquatic Genetic Resources, School
of Marine Science and Technology, Zhejiang Ocean University, Zhoushan 316022, China Received 24 December 2019; accepted 14 February 2020 © Chinese Society for Oceanography and Springer-Verlag GmbH Germany, part of Springer Nature 2020
Abstract
As an economically important marine fish, the large yellow croaker Larimichthys crocea suffered from marine white spot disease caused by the ectoparasite Cryptocaryon irritans in recent years. This disease not only could result in physiological damage, but also lead to secondary bacterial invasion. Reports indicated some AMPs (antimicrobial peptides) were of antiparasitic activity to C. irritans. Hepcidin-like (Lc-HepL) was one of the significant differential expression genes excavated from the transcriptome following a challenge with C. irritans. In this study, we characterized this AMP’s bioactivity based on the levels of mRNA and protein. After challenged by C. irritans, qRT-PCR showed Lc-HepL was significantly upregulated in six tissues, including gill, muscle, liver, head kidney and spleen during theront infection, trophont falling off, and secondary bacterial invasion stages, which implicated a role Lc-HepL played in the immune defense against C. irritans and secondary bacterial infection. Recombinant Lc-HepL (rLc-HepL) was induced and purified successfully. rLc-HepL exhibited antibacterial activity to certain bacteria in a dose- and time-dependent manners. Anti-C. irritans activity was explored for the first time and found it could cause the theronts membrane rupture and contents leakage. These results provided the first evidence that Lc-HepL had strong antiparasitic activity against marine fish ectoparasites C. irritans theronts. Together, data indicated that Lc-HepL might be an important component in the innate immune system against C. irritans and has the potential to be employed in future drug development. Key words: Larimichthys crocea, Cryptocaryon irritans, hepcidin-like, antibacterial activity, antiparasitic activity Citation: Zheng Libing, Li Yuan, Wang Jun, Pan Ying, Chen Jia, Zheng Weiqiang, Lin Longshan. 2020. Antibacterial and antiparasitic activities analysis of a hepcidin-like antimicrobial peptide from Larimichthys crocea. Acta Oceanologica Sinica, 39(10): 129–139, doi: 10.1007/s13131-020-1580-6
1 Introduction AMPs (antimicrobial peptides) are the central components in the humoral immunity of the innate immune system involving in the first defense line, and are either induced after being infected by pathogens or stored in the secretory cells (Valero et al., 2020). AM
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