Antibacterial assay guided isolation of a novel hydroxy-substituted pentacyclo ketonic compound from Pseudomonas aerugin
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ENVIRONMENTAL MICROBIOLOGY - RESEARCH PAPER
Antibacterial assay guided isolation of a novel hydroxy-substituted pentacyclo ketonic compound from Pseudomonas aeruginosa MBTDCMFRI Ps04 Anusree V. Nair 1
&
Praveen N. K 2 & Leo Antony. M 3 & K. K. Vijayan 3
Received: 3 May 2020 / Accepted: 12 November 2020 # Sociedade Brasileira de Microbiologia 2020
Abstract Antimicrobial compounds from the natural source have gained greater relevance because of their wide spectrum of possible applications, especially in the aquaculture industry where pathogenic threat and antibacterial resistance are serious concerns. In this regard, Pseudomonas aeruginosa MBTDCMFRI Ps04 (P. aeruginosa Ps04) strain isolated from the tropical estuarine habitats of Cochin was evaluated for its antibacterial potential against major aquaculture pathogens. The physiological conditions for the maximum production of the active metabolite were also optimized. An activity-guided approach was employed further to isolate and characterize the secondary metabolite responsible for the inhibitory potential. It was found that the cell free supernatant (CFS) of P. aeruginosa Ps04 exhibited strong antibacterial activity against major aquaculture pathogens belonging to the genus Vibrio and Aeromonas and retained its potential even at 30% (v/v) dilution. The highest antibacterial activity was detected from 3rd day culture, grown in glycerol alanine media (1% each) as carbon and nitrogen source, respectively, at 30 °C, pH 7.0 and at a salinity of 20 parts per thousand (ppt). The activity of the antagonistic principle was found to be stable against variations in pH (pH 2–pH 12), temperature (up to 120 °C) and enzymatic treatments. Bioassay-guided purification followed by spectroscopic characterization of active fractions of P. aeruginosa Ps04 revealed that the compound 4-Hydroxy-11-methylpentacyclo [11.8.0.02,3.011, 12.016,17]henicosa-1,3,5,8(9),17-penten-14-one is responsible for its major antibacterial activity. The results of this study indicated that P. aeruginosa Ps04 has beneficial antibacterial properties which could be used in developing novel antimicrobial therapeutics against a variety of aquaculture pathogens. Keywords P. aeruginosa Ps04 . Antibacterial compound . FT-IR, LC-MS, NMR
Introduction Bacterial disease outbreaks are considered a major threat which adversely affects the aquaculture industry by infecting almost all animals irrespective of their size and environment. Antibiotics and other chemicals are generally used to control
Responsible Editor: Fernando R. Pavan * K. K. Vijayan [email protected] 1
ICAR - Central Marine Fisheries Research Institute, Kochi, Kerala, India
2
Department of Chemistry, NSS College Cherthala, Cherthala, Kerala, India
3
ICAR - Central Institute of Brackish Water Aquaculture, Chennai, India
or prevent such infectious diseases. However, the use of antibiotics leads to generate antimicrobial resistance in host animals and other environmental related issues [1, 2]. The use of probiotic organisms was considered an ef
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