Isolation and Characterization of a Novel myovirus Infecting Shigella dysenteriae from the Aeration Tank Water
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Isolation and Characterization of a Novel myovirus Infecting Shigella dysenteriae from the Aeration Tank Water Han Lu 1 & Honghui Liu 1 & Min Lu 1 & Jingwei Wang 1 & Xinchun Liu 1 & Ruyin Liu 1 Received: 24 October 2019 / Accepted: 12 March 2020/ # Springer Science+Business Media, LLC, part of Springer Nature 2020
Abstract
The genome sequence, morphology, and genetic features of a novel phage, named SSE1, is reported here. Phage SSE1 that infects Shigella dysenteriae (China General Microbiological Culture Collection Center number: 1.1869) was isolated from the aeration tank water of a sewage treatment plant. SSE1 showed morphological features associated with those of phages in Myoviridae. The whole genome sequence of phage SSE1 is composed of 169,744 bp with the GC content of 37.51%. The double-stranded DNA of SSE1 contains 270 open reading frameworks (ORFs). Phylogenetically, phage SSE1 showed a stronger homology (whole genome and terminase large subunit protein sequence) to Escherichia phages than other Shigella phages in the NCBI database, but SSE1 did not infect Escherichia stains. This indicates that phage SSE1 should be a novel phage infecting Shigella dysenteriae. Besides, the result of this study provided a new idea for phage therapy. SSE1 may become a candidate for potential therapy against Shigella dysenteriae infection in clinical applications. Keywords Shigella dysenteriae . Phage . Isolation . Genome
Introduction Many Shigella strains are pathogens, disseminated through water and food [1–5]. In 2016, 12,597 cases of culture-confirmed Shigella infections were reported by 52 state and regional Electronic supplementary material The online version of this article (https://doi.org/10.1007/s12010-02003310-0) contains supplementary material, which is available to authorized users.
* Xinchun Liu [email protected] * Ruyin Liu [email protected]
1
College of Resources and Environment, University of Chinese Academy of Sciences, Beijing 101408, China
Applied Biochemistry and Biotechnology
public health laboratories to Laboratory-based Enteric Disease Surveillance (LEDS) [6]. S. dysenteriae is a diarrheal bacterium that infects 80–165 million people and kills an estimated 600,000 people worldwide each year [7, 8]. In general, Shigella infection can be treated with antibiotics. But, multidrug-resistant and all-drug–resistant super bacteria continue to gush out because of the abuse of antibiotics [9]. It is estimated that there are 27,000 drug-resistant Shigella infections occurring in the USA each year [10]. Super bacterial infections occur quickly, and the available antibiotics are limited, so phage therapy has been paid more and more attention [11]. In recent years, there are many successful cases of phage therapy reported [12, 13]. For example, phage therapy cured a 68-year-old diabetic patient with necrotizing pancreatitis complicated by a multidrug-resistant Acinetobacter baumannii infection in the USA [14]. Host specificity of phage can deal with bacterial infection very well [15]. Phage therapy has a
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