Antibiotic resistance assessment of Acinetobacter baumannii isolates from Tehran hospitals due to the presence of efflux
- PDF / 1,230,876 Bytes
- 6 Pages / 595.276 x 790.866 pts Page_size
- 45 Downloads / 193 Views
C Research Notes Open Access
RESEARCH NOTE
Antibiotic resistance assessment of Acinetobacter baumannii isolates from Tehran hospitals due to the presence of efflux pumps encoding genes (adeA and adeS genes) by molecular method Batool Basatian‑Tashkan1, Mohammad Niakan1*, Mansoor Khaledi1, Hamed Afkhami1, Fatemeh Sameni1, Shahriar Bakhti1 and Reza Mirnejad2
Abstract Objective: Acinetobacter baumannii (A. baumannii) has caused many problems in nosocomial infections. Efflux pumps are considered as one of the most important mechanisms of resistance in this bacterium and have the ability to excrete toxic substances such as antibiotics out of the cell. Results: In this study, 60 isolates of A. baumannii were collected from patients in several hospitals in Tehran, Iran. After diagnosis using standard biochemical methods, the pattern of antibiotic susceptibility was determined using the disk diffusion method according to CLSI guidelines. The adeA and adeS genes were identified by PCR method. The highest resistance to Piperacillin and the lowest resistance to Gentamicin were observed (100% compared to 48.4%). 6.6% of the isolates had only adeA gene and adeS gene was observed in 8.4% of isolates and both genes were detected in 73.4% of the samples. Despite the high resistance of t A. baumannii o antibiotics and due to the high fre‑ quency of genes of adeA and adeS efflux pumps in A. baumannii isolates, it can be concluded that these efflux pumps may play an important role in resistance of this bacterium. By determining the pattern of antibiotic the resistance before treatment, the resistance of this pathogen can be prevented in societies. Keywords: Acinetobacter baumannii, Efflux pump, adeA gene, adeS gene, Antibiotic resistance Introduction Nosocomial infections are known as one of the crucial problems for public health [1]. Most of these infections are caused by Gram-negative bacilli [2]. One of the most important causes of nosocomial infections is A. baumannii that is Gram-negative Coccobacilli and aerobic and prefer humid environments for a living [3, 4]. The *Correspondence: [email protected] 1 Department of Microbiology, Faculty of Medicine, Shahed University, Tehran, Iran Full list of author information is available at the end of the article
most important pathogen of this genus is, A. baumannii which can cause a wide range of diseases and nosocomial infections such as pneumonia, septicemia, urinary tract infections, skin and wound infections, endocarditis, and meningitis [5, 6]. Infection with this bacterium is increasing in people with immunodeficiency or cancer [7]. One of the main problems caused by this bacterium is the development of high antibiotic resistance [8]. One of the mechanisms that this bacterium uses to resist various antibiotics is the use of efflux pumps. By using efflux pumps, the A. baumannii can direct antibiotics outwards and prevent antibiotics from affecting the bacteria [9].
© The Author(s) 2020. This article is licensed under a Creative Commons Attribution 4.0 International License,
Data Loading...
