Acinetobacter baumannii Methods and Protocols
This detailed volume serves clinicians and basic science researchers studying the increasingly antibiotic resistant Gram-negative bacterium Acinetobacter baumannii. Chapters detail microbiological techniques, biochemical techniques, clinical samples, and
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Indranil Biswas Philip N. Rather Editors
Acinetobacter baumannii Methods and Protocols
METHODS
IN
MOLECULAR BIOLOGY
Series Editor John M. Walker School of Life and Medical Sciences University of Hertfordshire Hatfield, Hertfordshire, AL10 9AB, UK
For further volumes: http://www.springer.com/series/7651
Acinetobacter baumannii Methods and Protocols
Edited by
Indranil Biswas Department of Microbiology, Molecular Genetics and Immunology, University of Kansas Medical Center, Kansas City, KS, USA
Philip N. Rather Department of Microbiology and Immunology, Emory University, Atlanta, GA, USA
Editors Indranil Biswas Department of Microbiology Molecular Genetics and Immunology University of Kansas Medical Center Kansas City, KS, USA
Philip N. Rather Department of Microbiology and Immunology Emory University Atlanta, GA, USA
ISSN 1064-3745 ISSN 1940-6029 (electronic) Methods in Molecular Biology ISBN 978-1-4939-9117-4 ISBN 978-1-4939-9118-1 (eBook) https://doi.org/10.1007/978-1-4939-9118-1 Library of Congress Control Number: 2018968407 © Springer Science+Business Media, LLC, part of Springer Nature 2019 This work is subject to copyright. All rights are reserved by the Publisher, whether the whole or part of the material is concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfilms or in any other physical way, and transmission or information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed. The use of general descriptive names, registered names, trademarks, service marks, etc. in this publication does not imply, even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. The publisher, the authors, and the editors are safe to assume that the advice and information in this book are believed to be true and accurate at the date of publication. Neither the publisher nor the authors or the editors give a warranty, express or implied, with respect to the material contained herein or for any errors or omissions that may have been made. The publisher remains neutral with regard to jurisdictional claims in published maps and institutional affiliations. This Humana Press imprint is published by the registered company Springer Science+Business Media, LLC, part of Springer Nature. The registered company address is: 233 Spring Street, New York, NY 10013, U.S.A.
Preface Acinetobacter baumannii is a Gram-negative bacterium that has become a serious human pathogen. This bacterium is responsible for infections of the lung, skin/soft tissue, bloodstream, and urinary tract. Although A. baumannii is primarily an opportunistic pathogen, infections in the community have been increasing steadily, and this worrisome event suggests that strains with enhanced virulence have arisen. The ability to treat A. baumannii infections has become challenging due to the extensive
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