Molecular Characterization of Carbapenem-Resistant Acinetobacter baumannii Associated with Nosocomial Infection in the P
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Molecular Characterization of Carbapenem‑Resistant Acinetobacter baumannii Associated with Nosocomial Infection in the Pelotas, RS, Brazil Stella Buchhorn de Freitas1,2 · Suélen Cavalheiro Amaral1,2 · Marcos Roberto A. Ferreira1 · Bárbara Couto Roloff1,2 · Clóvis Moreira Jr.1 · Fabricio Rochedo Conceição1 · Daiane Drawanz Hartwig1,2 Received: 6 January 2020 / Accepted: 26 May 2020 © Springer Science+Business Media, LLC, part of Springer Nature 2020
Abstract Acinetobacter calcoaceticus–Acinetobacter baumannii complex (ACB) comprises some opportunistic pathogens associated with infectious outbreaks in hospital settings. A. baumannii is the most relevant species owing to its capacity to develop resistance to the different classes of antimicrobials. The aim of this study was to identify the species, establish the genetic patterns, resistance and biofilm profiles in ACB isolates associated with nosocomial infection in a hospital of Pelotas, Rio Grande do Sul, Brazil. Twenty-two clinical isolates were characterized at the species level through multiplex polymerase chain reaction (PCR) for the gyrB and blaOXA51-like genes, and the genetic relationship was determined through pulsed-field gel electrophoresis (PFGE). Their antibiotic resistance profiles and carbapenemases synthesis were evaluated following CLSI guidelines. PCR was carried out to evaluate the presence of carbapenemases genes and the isolates were classified for their biofilm-forming ability. All isolates obtained in the study were identified as A. baumannii and 72.7% of the isolates were classified as strong biofilm formers. In the class carbapenems, 95.4% and 77.3% of the isolates were resistant to meropenem and imipenem, respectively. The blaVIM gene was identified in 90.9% of isolates and carbapenemases synthesis were confirmed in 95.4% of the isolates. Fourteen genetic patterns were confirmed through PFGE analyses. The isolates collected within a time gap of 2 years demonstrated a genetic relationship, and the same clone was identified in different departments in the hospital. To the best of our knowledge, this is the first report of identification and characterization of A. baumannii nosocomial isolates in Pelotas, RS, Brazil.
Introduction The genus Acinetobacter comprises a group of Gram-negative coccobacilli, which are catalase-positive, oxidase-negative, non-motile and non-fermenting, belonging to the family Moraxellaceae and order Gammaproteobacteria [1, 2]. More than 15 species of this genus are clinically relevant because they are considered opportunistic pathogens in humans [3]. Due to the phenotypic similarities and the close genetic relationship, A. baumannii, A. nosocomialis, A. pittii, and A. * Daiane Drawanz Hartwig [email protected] 1
Center of Technological Development, Biotechnology, Federal University of Pelotas, Pelotas, Rio Grande do Sul, Brazil
Department of Microbiology and Parasitology, Biology Institute, Federal University of Pelotas, University Campus, mailbox 354, Pelotas, RS CEP 96010–900, Brazil
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calcoaceticus
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