Anticancer activity of Turkish marine extracts: a purple sponge extract induces apoptosis with multitarget kinase inhibi
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PRECLINICAL STUDIES
Anticancer activity of Turkish marine extracts: a purple sponge extract induces apoptosis with multitarget kinase inhibition activity Halil I. Ciftci 1,2 & Mustafa Can 2,3 & Doha E. Ellakwa 2,4 & Salih C. Suner 5 & Mohamed A. Ibrahim 6 & Ayhan Oral 7 & Nazim Sekeroglu 8 & Barış Özalp 9 & Masami Otsuka 1,2 & Mikako Fujita 2 & Mustafa Alparslan 10 & Mohamed O. Radwan 1,2,6 Received: 6 January 2020 / Accepted: 10 February 2020 # Springer Science+Business Media, LLC, part of Springer Nature 2020
Summary Marine natural products have drawn a great deal of attention as a vital source of new drugs for the last five decades. However, marine organisms in the seas surrounding Turkey (the Black Sea, the Aegean Sea and the Mediterranean Sea) haven’t been yet extensively explored. In the present study, three marine organisms (Dysidea avara, Microcosmus sabatieri and Echinaster sepositus) were sampled from the Dardanelles (Turkish Straits System, Western Turkey) by scientific divers, transferred to the laboratory and then were extracted with 70% ethanol. The extracts were tested for their cytotoxic effect against K562, KMS12PE, A549, and A375 cancer cell lines. The sponge extract elicited the most promising cytotoxic activity, thus it was further evaluated against H929, MCF-7, HeLa, and HCT116 cancer cells. Most of the designated cells showed a considerable sensitivity for the sponge extract particularly H929, K562, KMS-12PE and HeLa cells with IC50 less than 10 μg/mL. On the contrary, the other two extracts exhibited no cytotoxic activity on all cells at 100 μg/mL concentration. The sponge extract was tested for its capacity to induce apoptosis in cancer cells and to inhibit a panel of tyrosine kinases showing remarkable results. The outcome of this study represents a platform for discovery of new chemotherapeutic agents of marine natural origin. Keywords Marine products . Cancer . Apoptosis . Tyrosine kinase inhibitors
* Mikako Fujita [email protected]
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Department of Biochemistry and Molecular Biology, Faculty of Pharmacy, Al-Azhar University, Greater Cairo, Nasr City 11651, Egypt
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* Mohamed O. Radwan [email protected]; [email protected]
Chemical and Chemical Processing, Vocational School of Lapseki, Canakkale Onsekiz Mart University, 17800 Canakkale, Turkey
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Chemistry of Natural Compounds Department, Pharmaceutical and Drug Industries Research Division, National Research Centre, Dokki, Cairo 12622, Egypt
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Department of Drug Discovery, Science Farm Ltd., 1-7-30-805 Kuhonji, Chuo-ku, Kumamoto 862-0976, Japan
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Department of Chemistry, Faculty of Science, Canakkale Onsekiz Mart University, 17020 Canakkale, Turkey
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Medicinal and Biological Chemistry Science Farm Joint Research Laboratory, Faculty of Life Sciences, Kumamoto University, 5-1 Oe-honmachi, Chuo-Ku, Kumamoto 8620973, Japan
Department of Horticulture, Faculty of Agriculture, Kilis 7 Aralik University, 79000 Kilis, Turkey
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Section of Underwater Technology, Vocational School of Ocean Engineering, Ca
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