Anticontaminants to replace formaldehyde in an artificial diet used for rearing the sugarcane borer can affect the paras
- PDF / 283,637 Bytes
- 6 Pages / 595.276 x 790.866 pts Page_size
- 103 Downloads / 186 Views
ORIGINAL RESEARCH ARTICLE
Anticontaminants to replace formaldehyde in an artificial diet used for rearing the sugarcane borer can affect the parasitoids performance? Suélen Cristina da Silva Moreira 1
& Ivana Fernandes da Silva
1
& Crébio José Ávila
2
& Harley Nonato de Oliveira
2
Received: 9 December 2019 / Accepted: 14 May 2020 # African Association of Insect Scientists 2020
Abstract Formaldehyde has been widely used as an anticontaminant in artificial diets for rearing insects. However, the substance is highly toxic to humans. The objective of this work was to verify if replacing formaldehyde by acetylsalicylic acid, sorbic acid, or benzoic acid in artificial diet to use for rearing Diatraea saccharalis affect biological traits of its parasitoids Trichogramma galloi and Cotesia flavipes produced in this host species. The experimental design was the completely randomized with the following treatments (dose/diet): acetylsalicylic acid (3.7 g), sorbic acid (3.7 g), benzoic acid (3.7 g), formaldehyde (1.7 ml), and control. The studies of the biological characteristics of T. galloi and C. flavipes consisted of 25 replicates. For T. galloi, each replicate consisted of a mass of 30 eggs of D. saccharalis that were exposed for 24 h to a T. galloi female which was less than 24 h old. For C. flavipes, each replicate used a third instar D. saccharalis caterpillar oviposited by a C. flavipes female less than 24 h old. The biological characteristics evaluated were parasitism, emergence, progeny, sexual ratio and longevity. The use of alternative anticontaminants tested in the artificial diets of D. saccharalis is feasible for the production of T. galloi and C. flavipes parasitoids. Keywords Adaptation of artificial diet . Mass rearing . Standardization of breeding . Biological control . Sugarcane borer
Introduction Biological control, a tool that uses conservation and/or introduction of control agents, is an important component of Integrated Pest Management (IPM) in various crops around the world (Bebber et al. 2014; van Lenteren et al. 2018). In Brazil, the use of applied biological control has been increasing in recent years, mainly due to the evolution of insect rearing techniques in the laboratory (Togni et al. 2019). For example, the parasitoid species Cotesia flavipes (Cameron) (Hymenoptera: Braconidae) have been used to control Diatraea saccharalis (Fabricius) (Lepidoptera: Crambidae); Tamarixia radiata (Waterston) (Hymenoptera: Eulophidae) to control Diaphorina citri Kuwayama (Hemiptera:
* Ivana Fernandes da Silva [email protected] 1
Departamento de Entomologia e Conservação da Biodiversidade, Universidade Federal da Grande Dourados, Dourados, Mato Grosso do Sul, Brazil
2
Embrapa Agropecuária Oeste, Dourados, Mato Grosso do Sul, Brazil
Psyllidae); and Trichogramma spp. (Hymenoptera: Trichogrammatidae) to control various species of pest lepidopterans, in addition to the egg parasitoids used to control the Pentatomidae sinkbug complex (Oliveira et al. 2014; Aya et al. 2017; Laurentis et al. 2019;
Data Loading...
