Antiviral Resistance in Plants Methods and Protocols
Studies related to pathogen-mediated virus resistance in plants were instrumental in providing some of the historical observations which ultimately led to the vital discovery of double-stranded RNA (dsRNA)-induced gene silencing or RNA interference (RNAi)
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IN
MOLECULAR BIOLOGY™
Series Editor John M. Walker School of Life Sciences University of Hertfordshire Hatfield, Hertfordshire, AL10 9AB, UK
For further volumes: http://www.springer.com/series/7651
Antiviral Resistance in Plants Methods and Protocols Edited by
John M. Watson and Ming-Bo Wang Division of Plant Industry, CSIRO, Canberra, ACT, Australia
Editors John M. Watson Division of Plant Industry CSIRO Canberra, ACT, Australia
Ming-Bo Wang Division of Plant Industry CSIRO Canberra, ACT, Australia
ISSN 1064-3745 e-ISSN 1940-6029 ISBN 978-1-61779-881-8 e-ISBN 978-1-61779-882-5 DOI 10.1007/978-1-61779-882-5 Springer New York Dordrecht Heidelberg London Library of Congress Control Number: 2012938993 © Springer Science+Business Media, LLC 2012 All rights reserved. This work may not be translated or copied in whole or in part without the written permission of the publisher (Humana Press, c/o Springer Science+Business Media, LLC, 233 Spring Street, New York, NY 10013, USA), except for brief excerpts in connection with reviews or scholarly analysis. Use in connection with any form of information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed is forbidden. The use in this publication of trade names, trademarks, service marks, and similar terms, even if they are not identified as such, is not to be taken as an expression of opinion as to whether or not they are subject to proprietary rights. Printed on acid-free paper Humana Press is part of Springer Science+Business Media (www.springer.com)
Preface Studies related to pathogen-mediated virus resistance in plants throughout the 1990s were instrumental in providing some of the historical observations which ultimately led to the discovery of double-stranded RNA (dsRNA)-induced gene silencing or RNA interference (RNAi). The introductory chapter in this volume by John Lindbo details a historical overview of pathogen-mediated virus resistance research in plants. Together with his colleague William Dougherty, John carried out some of the seminal studies which initially revealed that transgene-mediated virus resistance in plants was the result of a sequence-specific degradation of viral RNA, providing the first piece of evidence for an RNA-based gene silencing mechanism in plants. These early observations, and the subsequent discovery of dsRNA-induced gene silencing, now commonly known as RNA silencing, have since revolutionized research on plant–virus interactions. Two other overview chapters are included in this volume for the benefit of newcomers to this relatively new area of RNA silencing-mediated antiviral defense in plants. The chapter by Aguis et al. provides a comprehensive overview of the various RNA silencing pathways in plants, with an emphasis on how RNA silencing is involved in plant–virus interactions and how RNA silencing can be exploited for engineering virus resistance in plants. A third overview by Omarov and Scholtof discusses viral counter defense mechanisms
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