Chromosome and Genomic Engineering in Plants Methods and Protocols
This volume assembles protocols for chromosome engineering and genome editing in two recently developed approaches for manipulating chromosomal and genomic DNA in plants. The first approach is a “plant chromosome vector” system, which allows the introduct
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Minoru Murata Editor
Chromosome and Genomic Engineering in Plants Methods and Protocols
METHODS
IN
MOLECULAR BIOLOGY
Series Editor John M. Walker School of Life and Medical Sciences University of Hertfordshire Hatfield, Hertfordshire, AL10 9AB, UK
For further volumes: http://www.springer.com/series/7651
Chromosome and Genomic Engineering in Plants Methods and Protocols
Edited by
Minoru Murata Institute of Plant Science and Resources, Okayama University, Kurashiki, Japan
Editor Minoru Murata Institute of Plant Science and Resources Okayama University Kurashiki, Japan
ISSN 1064-3745 ISSN 1940-6029 (electronic) Methods in Molecular Biology ISBN 978-1-4939-4929-8 ISBN 978-1-4939-4931-1 (eBook) DOI 10.1007/978-1-4939-4931-1 Library of Congress Control Number: 2016946367 © Springer Science+Business Media New York 2016 This work is subject to copyright. All rights are reserved by the Publisher, whether the whole or part of the material is concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfilms or in any other physical way, and transmission or information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed. The use of general descriptive names, registered names, trademarks, service marks, etc. in this publication does not imply, even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. The publisher, the authors and the editors are safe to assume that the advice and information in this book are believed to be true and accurate at the date of publication. Neither the publisher nor the authors or the editors give a warranty, express or implied, with respect to the material contained herein or for any errors or omissions that may have been made. Cover illustration: Arabidopsis transgenic plants in plate, expressing Ac transposase Printed on acid-free paper This Humana Press imprint is published by Springer Nature The registered company is Springer Science+Business Media LLC New York
Preface Transformation or transfection is an indispensable tool in basic and applied studies in biological sciences. In plants, a number of species can be transformed by an Agrobacteriummediated system, particle bombardment, and/or protoplast fusion. Compared to other organisms, however, these three techniques are uncontrollable with regard to the insertion of exogenous genes or DNA because the insertion into the genome or chromosome is quite random, and multiple-copy insertion frequently occurs. This random and multiple-copy insertion increases the risk of disrupting essential genes. To avoid such risk, gene targeting via homologous recombination is most desirable, as has been shown in yeast and mice. However, the occurrence of homologous recombination is quite limited in plants, except for in some lower plants (i.e., Physcomitrella patens and Chlamydomonas reinhard
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