Application of platelet-rich plasma in the in vitro production of bovine embryos
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Application of platelet-rich plasma in the in vitro production of bovine embryos Pamela Ramos-Deus 1 & Pábola Santos Nascimento 1 & Joane Isis Travassos Vieira 1 & Maiana Silva Chaves 1 Karoline Antunes Albuquerque 1 & José Carlos Ferreira-Silva 1 & João Grabriel Viana Grázia 2 & Antonio Santana Santos Filho 3 & André Mariano Batista 1 & Valéria Wanderley Teixeira 4 & Marcos Antonio Lemos Oliveira 1
&
Received: 19 January 2020 / Accepted: 20 May 2020 # Springer Nature B.V. 2020
Abstract The aim of this study was to replace fetal bovine serum (FBS) with platelet-rich plasma (PRP) for in vitro production of bovine embryos. The maturation media (TCM-199 medium) for the cumulus-oocyte complexes (COCs) was supplemented with 5% (G5) and 10% (G10) PRP or 10% FBS (GC). After fertilization, the presumed zygotes were randomly distributed in culture medium supplemented with 5% (G5) and 10% (G10) PRP or 10% FBS (GC) for 7 days. Cumulus cell (CC) expansion was greater (P < 0.05) in the GC (88.9%) group than in G5 (34.1%) or G10 (50.0%). Nevertheless, the expansion of CCs in group G10 was greater than in G5 (P < 0.05). Cleavage was higher in group G5 (86.0%) than in G10 (79.0%) (P < 0.05) and did not differ from group GC (82.0%). The percentage of blastocysts in group G5 (50.0%) was higher than in CG (40.2%) and G10 (34.2%) (P < 0.05). In addition, the number of blastomeres was higher in G5 (159.0 ± 4.18) than in GC (132.4 ± 4.11) and in G10 (127.1 ± 5.88) (P < 0.05). The addition of PRP into the oocytes maturation medium is not beneficial. On the other hand, the PRP addition into the embryo culture medium at 5% concentration is recommended where it increased the quantity and quality of in vitro–produced bovine embryos. Keywords Blastocyst . Cleavage . Growth factor . Oocyte
Introduction In vitro embryo production (IVP) allows improving the number of genetically superior animals, even from females with acquired infertility not genetic related (Dos Santos et al. 2019). The IVP biotechnology is composed by the in vitro oocyte maturation (IVM), fertilization (IVF), and subsequently embryo culture (IVC) (Lima et al. 2004). Based on IVP studies, it has been possible to better understand the events
* Pamela Ramos-Deus [email protected] 1
Laboratório de Biotécnicas Reprodutivas, Universidade Federal Rural de Pernambuco, Recife, PE, Brazil
2
Apoyar Biotecnologia da Reprodução Animal, Juiz de Fora, MG, Brazil
3
Instituto Agronômico de Pernambuco, Arcoverde, PE, Brazil
4
Universidade Federal Rural de Pernambuco, Recife, PE, Brazil
involved during the oocyte maturation and embryo development processes (Lima et al. 2006; Dey et al. 2012). IVP is also essential for the application of other assisted reproduction biotechniques related, including intracytoplasmic sperm injection, nuclear somatic cell transfer, and cloning (Sekhavati et al. 2018; Cortez et al. 2018; Santos et al. 2019). However, the results from IVP, related to both maturation and embryo fertilization, are lower than those obtained from in vivo e
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