Does serum cause lipid-droplet accumulation in bovine embryos produced in vitro , during developmental days 1 to 4?
- PDF / 538,436 Bytes
- 12 Pages / 595.276 x 790.866 pts Page_size
- 77 Downloads / 201 Views
EMBRYO BIOLOGY
Does serum cause lipid-droplet accumulation in bovine embryos produced in vitro , during developmental days 1 to 4? Melisa Candela Crocco & Diana Mabel Kelmansky & Marta Inés Mariano
Received: 20 April 2013 / Accepted: 15 July 2013 / Published online: 4 August 2013 # Springer Science+Business Media New York 2013
Summary Purpose Serum supplementation has shown to have beneficial effects on in vitro bovine embryo development. However, it is often assumed that serum supplementation may produce mitochondrial damage and this damage would generate lipid accumulation, a major obstacle for cryopreservation. The aim of the present study is to investigate the previous assumptions in early embryonic stages. Methods We considered in vitro produced bovine embryos from day 1 to 4 of development, which were grown in presence of serum from days 1, 2 or 3 or in absence of it. Electron transmission micrographs allowed us to quantify the area occupied by lipid droplets and by the different mitochondrial types to evaluate serum effect. Using confocal microscopy we analyzed mitochondrial activity and location. Results We found no evidence of lipid droplets accumulation or mitochondrial degeneration or reduction of mitochondrial area in serum supplemented media. Further, our results suggest that events of mitochondrial proliferation are taking place even in serum supplemented media.
Capsule The present work is an experimental presentation that studies the effects of in vitro culture, on mammal’s embryo structure. It explains why better culture could be obtained by adding serum to the medium to a strict "in vitro" embryo age. Team Head was Marta Inés Mariano M. C. Crocco (*) : M. I. Mariano Instituto Nacional de Parasitología “M. F. Chaben” ANLIS Malbrán, Av. Paseo Colón 568, Ciudad de Buenos Aires, Argentina e-mail: [email protected] M. C. Crocco : M. I. Mariano Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Buenos Aires, Argentina D. M. Kelmansky Instituto de Cálculo. Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina
Conclusions Serum does not produce lipid accumulation or mitochondrial damage in bovine embryos from 2 to 16 cells. When serum was added to embryo culture medium on day 3 of development, there were ultrastructural signs of a beneficial effect for embryo development. The lack of serum until day 3 may also avoid the unnecessary exposure to potentially inhibitory factors present on it. Keywords Embryo . Serum . Lipid droplet . Mitochondria . Ultrastructure
Introduction Since the beginning of assisted reproductive technologies in cattle breeding, serum has regularly been used during part or throughout total bovine embryo in vitro development as source of energetic substrates and growth factors [29,38]. However, its complex undefined and variable composition may be associated with several subcellular changes such as lipid accumulation [2,51,52,57]. Cytoplasmic lipids are not only important nutrients for the developing embryo but they also m
Data Loading...
