Application of solidified floating double-solvent dispersive liquid-phase microextraction for the analysis of the main a
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Application of solidified floating double‑solvent dispersive liquid‑phase microextraction for the analysis of the main active components in Zicao Chengqi decoction Xiang Geng1,2 · Xuan Chen1 · Zhuoyi Li1 · Xiaohong Bai1 · Shuang Hu1 Received: 9 July 2020 / Accepted: 17 September 2020 © Springer Nature Switzerland AG 2020
Abstract A new method was established using solidified floating double-solvent dispersive liquid-phase microextraction combined with high-performance liquid chromatography (HPLC) for the simultaneous determination and extraction of six active ingredients from Aurantii Fructus Immaturus, Magnoliae Officinalis Cortex, Rhei Radix et Rhizoma, and Arnebiae Radix in the Zicao Chengqi decoction. The six active ingredients in Zicao Chengqi decoction include naringenin, honokiol, shikonin, magnolol, emodin, and β,β-dimethylacrylshikonin. In this experiment, the effects of the extractant type and volume, sample phase volume, sample phase pH, extraction time, and stirring speed on the enrichment factor were studied, and optimal extraction conditions were determined. Naringenin (6–600 ng/mL), honokiol (3–219 ng/mL), shikonin (4–350 ng/mL), magnolol (3–230 ng/mL), emodin (3–190 ng/mL), and β,β-dimethylacrylshikonin (3–430 ng/mL) had a good linear relationship and all the correlation coefficients were 0.9900–0.9992. This method was used for the simultaneous concentration and enrichment of the six active ingredients from the Chinese herbal compound, Zicao Chengqi decoction, with recoveries between 95.7 and 100.3%. The results showed that solidified floating double-solvent dispersive liquid-phase microextraction combined with HPLC can be used for the simultaneous extraction and analysis of multiple active components in the Zicao Chengqi decoction. Keywords High-performance liquid chromatography · Solidified floating organic drop dispersive liquid-phase microextraction · Zicao chengqi decoction
1 Introduction The Zicao Chengqi decoction, from Vol. 6 of the Zheng Zhi Zhun Sheng-you Ke, is prepared from four traditional Chinese herbs, including Aurantii Fructus Immaturus, Magnoliae Officinalis Cortex, Rhei Radix et Rhizoma, and Arnebiae Radix. It is a famous Chinese herbal medicine that relieves heat and removes toxins. At present, as a formula, the
efficacy and side effects of the Zicao Chengqi decoction have not been systematically assessed, although some studies have investigated the efficacy of the active compound.[1]. According to previous studies, emodin in Rhei Radix et Rhizoma also displays antitumor, anti-inflammatory, and antibacterial activity [2–4]. Shikonin and β,βdimethylacrylshikonin in Arnebiae Radix have been shown to exhibit many biological and pharmacological activities,
Co-first author: Professor Xuan Chen. Electronic supplementary material The online version of this article (https://doi.org/10.1007/s42452-020-03557-6) contains supplementary material, which is available to authorized users. * Xuan Chen, [email protected] | 1School of Pharmacy, Shanxi Medical University, Jinzhong 030619, Chi
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