Articular Cartilage Regeneration Utilizing Decellularized Human Placental Scaffold, Mesenchymal Stem Cells and Platelet
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Online ISSN 2212-5469
ORIGINAL ARTICLE
Articular Cartilage Regeneration Utilizing Decellularized Human Placental Scaffold, Mesenchymal Stem Cells and Platelet Rich Plasma ¨ zdemir1 • Abdu¨lsamet Emet1 • Ramin Hashemihesar2 • Ali Celalettin Sinan Yu¨ru¨ker3 • Erdi O Emine Kılıc¸4 • Duygu Uc¸kan C ¸ etinkaya4 • Egemen Turhan1
Received: 28 July 2020 / Revised: 10 August 2020 / Accepted: 26 August 2020 Ó The Korean Tissue Engineering and Regenerative Medicine Society 2020
Abstract BACKGROUND: Articular cartilage repair has been a challenge in orthopedic practice due to the limited self-regenerative capability. Optimal treatment method for cartilage defects has not been defined. We investigated the effect of decellularized human placental (DHP) scaffold, mesenchymal stem cells (MSC) and platelet-rich plasma (PRP) on hyaline cartilage regeneration in a rat model. METHODS: An osteochondral defect was created in trochlea region of the femur in all groups, bilaterally. No additional procedure was performed in control group (n = 14). Only the DHP scaffold was applied to the P group (n = 14). The DHP scaffold and 1 9 106 MSCs were applied to the PS group (n = 14). The DHP scaffold and PRP were applied to the PP group (n = 14). The DHP scaffold, 1 9 106 MSCs and PRP were applied to the PSP group (n = 14). Outcome measures at 12 weeks included Pineda histology score and qualitative histology. RESULTS: The mean Pineda scores of P, PS, PP, and PSP groups were significantly better than the control group (p = 0.031, p = 0.002, p \ 0.001, p \ 0001, respectively). There was no statistically difference in mean Pineda scores of P, PS, PP, and PSP groups (p [ 0.05). CONCLUSION: In conclusion, the DHP scaffold appears to be a promising scaffold on hyaline cartilage regeneration. The augmentation of DHP scaffold with MSCs and PRP combinations did not enhance its efficacy on articular cartilage regeneration. Keywords Cartilage Placenta decellularization Platelet-rich plasma Mesenchymal stem cells
1 Introduction This study was presented at the 29th National Congress of Turkish Orthopedics and Traumatology, October 22–27, 2019, in Antalya, Turkey. ¨ zdemir & Erdi O [email protected] 1
Department of Orthopedics and Traumatology, Faculty of Medicine, Hacettepe University, 06230 Ankara, Turkey
2
Department of Histology and Embryology, Faculty of Medicine, Istanbul Aydin University, 34295 Istanbul, Turkey
3
Department of Histology and Embryology, Faculty of Medicine, Us¸ ak University, 64000 Us¸ ak, Turkey
4
Center for Stem Cell Research and Development, Hacettepe University, 06100 Ankara, Turkey
Articular cartilage defects are a common problem in orthopedic practice. These defects mostly occur due to trauma or degenerative process [1]. They result in joint pain and degeneration which lead to decreased function at the associated extremity [2]. Treatment options of articular cartilage defects include debridement, osteochondral graft transfers, autologous chondrocyte implantation (ACI), and bone marrow stimulation te
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