Association analysis of HSP70A1A haplotypes with heat tolerance in Chinese Holstein cattle
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ORIGINAL PAPER
Association analysis of HSP70A1A haplotypes with heat tolerance in Chinese Holstein cattle Qi Xiong & Jin Chai & Haiqian Xiong & Wengong Li & Tao Huang & Yang Liu & Xiaojun Suo & Nian Zhang & Xiaofeng Li & Siwen Jiang & Mingxin Chen
Received: 19 December 2012 / Revised: 16 March 2013 / Accepted: 18 March 2013 # Cell Stress Society International 2013
Abstract Single-nucleotide polymorphisms (SNPs) in the coding and untranslated regions of heat shock 70 kDa protein 1A (HSP70A1A), an inducible molecular chaperone that is responsible for cellular protection against heat stress, have been reported as being associated with heat tolerance. A fragment of the HSP70A1A gene was amplified in Chinese Holstein cattle and eight novel mutations were found. We performed comprehensive linkage disequilibrium (LD) and haplotype analyses of the eight SNPs of the HSP70A1A gene and examined their involvement in heat resistance in 600 Chinese Holstein cattle. Our results revealed the presence of significant differences between individuals carrying haplotype 1 and those without haplotype 1 for most of the heat-tolerance traits. Haplotype 1 increased the risk of heat stress; however, association analysis of its combination with haplotype 2 showed the lowest rectal temperature and red blood cell K+ level, moderate respiratory rate, and the Q. Xiong : Y. Liu : X. Suo : N. Zhang : X. Li : M. Chen (*) Hubei Key Laboratory of Animal Embryo Engineering and Molecular Breeding, Institute of Animal Husbandry and Veterinary, Hubei Academy of Agricultural Sciences, Wuhan 430064, China e-mail: [email protected] H. Xiong : W. Li Institute of Animal Husbandry and Veterinary, Huanggang Academy of Agricultural Sciences, Huanggang 438000, China T. Huang Animal Husbandry and Veterinary Bureau of Hubei Province, Wuhan 430064, China J. Chai : S. Jiang Agricultural Ministry Key Laboratory of Swine Breeding and Genetics & Key Laboratory of Agricultural Animal Genetics, Breeding, and Reproduction of Ministry of Education, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan 430070, China
highest red blood cell NKA level, suggesting a heterozygote advantage in the penetration of the phenotype. Protein expression levels in white blood cells among haplotype combinations further confirmed the hypothesis that heterozygotes for haplotypes 1 and 2 are more sensitive to heat stress. We presume that these mutations may be useful in the future as molecular genetic markers to assist selection for heat tolerance in cattle. Keywords HSP70 . Heat stress . Haplotypes . Cattle . Association analysis
Introduction Chinese Holstein is a heat-sensitive breed of cattle that originated in Europe. High environmental temperature exerts a negative influence on the performance of Chinese Holstein cattle, especially in South China. In summer, the high temperature and humidity can produce moderate heat stress, resulting in decreased dry-matter intake, immunity, milk secretion, and reproduction performance (Wolfenson et al. 2000; Berman 201
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