Genome-wide association analysis of stripe rust resistance in modern Chinese wheat
- PDF / 1,914,364 Bytes
- 13 Pages / 595.276 x 790.866 pts Page_size
- 68 Downloads / 162 Views
RESEARCH ARTICLE
Open Access
Genome-wide association analysis of stripe rust resistance in modern Chinese wheat Mengjie Jia1,2, Lijun Yang3, Wei Zhang4, Garry Rosewarne5,6, Junhui Li1, Enian Yang7, Ling Chen1, Wenxue Wang1, Yike Liu1, Hanwen Tong1, Weijie He1, Yuqing Zhang1, Zhanwang Zhu1* and Chunbao Gao1,8*
Abstract Background: Stripe rust (yellow rust) is a significant disease for bread wheat (Triticum aestivum L.) worldwide. A genome-wide association study was conducted on 240 Chinese wheat cultivars and elite lines genotyped with the wheat 90 K single nucleotide polymorphism (SNP) arrays to decipher the genetic architecture of stripe rust resistance in Chinese germplasm. Results: Stripe rust resistance was evaluated at the adult plant stage in Pixian and Xindu in Sichuan province in the 2015–2016 cropping season, and in Wuhan in Hubei province in the 2013–2014, 2016–2017 and 2018–2019 cropping seasons. Twelve stable loci for stripe rust resistance were identified by GWAS using TASSEL and GAPIT software. These loci were distributed on chromosomes 1B, 1D, 2A, 2B, 3A, 3B, 4B (3), 4D, 6D, and 7B and explained 3.6 to 10.3% of the phenotypic variation. Six of the loci corresponded with previously reported genes/QTLs, including Sr2/Yr30/Lr27, while the other six (QYr.hbaas-1BS, QYr.hbaas-2BL, QYr.hbaas-3AL, QYr.hbaas-4BL.3, QYr.hbaas4DL, and QYr.hbaas-6DS) are probably novel. The results suggest high genetic diversity for stripe rust resistance in this population. The resistance alleles of QYr.hbaas-2AS, QYr.hbaas-3BS, QYr.hbaas-4DL, and QYr.hbaas-7BL were rare in the present panel, indicating their potential use in breeding for stripe rust resistance in China. Eleven pentaprimer amplification refractory mutation system (PARMS) markers were developed from SNPs significantly associated with seven mapped QTLs. Twenty-seven genes were predicted for mapped QTLs. Six of them were considered as candidates for their high relative expression levels post-inoculation. Conclusion: The resistant germplasm, mapped QTLs, and PARMS markers developed in this study are resources for enhancing stripe rust resistance in wheat breeding. Keywords: Marker-trait association, Single nucleotide polymorphism (SNP), Triticum aestivum, Yellow rust
Background Stripe rust (yellow rust), incited by obligate biotroph fungus Puccinia striiformis Westend. f. sp. tritici (Pst), is a significant disease of bread wheat (Triticum aestivum L.) worldwide. An outbreak will fast destroy green leaves and, in turn, dramatically reduce photosynthesis, resulting in * Correspondence: [email protected]; [email protected] 1 Hubei Key Laboratory of Food Crop Germplasm and Genetic Improvement, Food Crops Institute, Hubei Academy of Agricultural Sciences/Hubei Engineering and Technology Research Center of Wheat/Wheat Disease Biology Research Station for Central China, Wuhan 430064, China Full list of author information is available at the end of the article
stunted and weakened plants, reduced grain numbers per spike, shriveled grains, and lower grain weights. G
Data Loading...
