Association between long-chain non-coding RNA SRA1 gene single-nucleotide polymorphism and polycystic ovary syndrome sus
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GENETICS
Association between long-chain non-coding RNA SRA1 gene single-nucleotide polymorphism and polycystic ovary syndrome susceptibility. Jifan Tan 1
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XiuLan Hao 1 & TingTing Zhao 1 & JianLan Ying 1 & Tian Li 1 & Li Cheng 1
Received: 10 June 2020 / Accepted: 6 August 2020 # Springer Science+Business Media, LLC, part of Springer Nature 2020
Abstract Objective To study the association between single-nucleotide polymorphism (SNP) of long-chain non-coding RNA steroid receptor RNA activator (lncRNA SRA1) gene and polycystic ovary syndrome (PCOS) susceptibility. Methods Sanger sequencing was used to analyze the genotypes of the lncRNA SRA1 gene rs801460, rs10463297, and rs250426 in 315 PCOS patients and 315 control groups. Results There was no correlation between lncRNA SRA1 gene rs801460, rs250426 SNP, and PCOS susceptibility (p > 0.05). The T allele at the rs10463297 locus of the SRA1 gene has a lower risk of PCOS than the C allele (OR = 0.63, 95%CI: 0.50–0.79, p < 0.01). Among people with a BMI ≥ 26.5 kg/m2, when carrying the TC genotype and CC genotype at rs801460, the risk of PCOS susceptibility was lower than the TT genotype (OR = 0.54, 95%CI: 0.33–0.89, p = 0.02). At different ages and BMI stratifications, there was a significant association between rs10463297 SNP and PCOS susceptibility (p < 0.05). Multi-factor dimensionality reduction (MDR) analysis results showed that age, BMI, rs801460, rs10463297, and rs250426 interactions constitute a “high-risk combination.” PCOS susceptibility risk was 5.96 times that of a “low-risk combination” (95%CI: 4.14– 8.56, p < 0.01). SRA1 gene rs801460, rs10463297, rs250426 constructed TCT haplotype was associated with increased risk of PCOS susceptibility (OR = 1.66, 95%CI: 1.20–2.30, p < 0.01); the CTT haplotype was associated with a decreased risk of PCOS susceptibility (OR = 0.56, 95%CI: 0.36–0.87, p = 0.01). LncRNA SRA1 gene rs10463297 SNP was correlated with the level of lncRNA SRA1 in the peripheral blood leukocytes (p < 0.01). Conclusion From this study, we found that the lncRNA SRA1 gene rs10463297 SNP is associated with PCOS susceptibility. Keywords Polycystic ovary syndrome . Single-nucleotide polymorphism . Steroid receptor RNA activator . Long noncoding RNA Jifan Tan and XiuLan Hao contributed equally to this work. * Tian Li [email protected] * Li Cheng [email protected] Jifan Tan [email protected] XiuLan Hao [email protected] TingTing Zhao [email protected] JianLan Ying [email protected] 1
Department of Obstetrics and Gynecology, The Seventh Affiliated Hospital of Sun Yat-sen University, Shenzhen 518107, China
Introduction Polycystic ovary syndrome (PCOS) is one of the most common reproductive endocrine diseases of women of childbearing age. The prevalence of women of childbearing age in the world is as high as 10%, and it is the most common cause of anovulatory infertility [1]. The main clinical manifestations of PCOS are rare ovulation or anovulation, polycystic ovarian changes, and clinical or laboratory hyperandrogenism [2]. Studies have shown that PCOS i
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