B7-H6, an immunoligand for the natural killer cell activating receptor NKp30, reveals inhibitory effects on cell prolife
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RESEARCH ARTICLE
Open Access
B7-H6, an immunoligand for the natural killer cell activating receptor NKp30, reveals inhibitory effects on cell proliferation and migration, but not apoptosis, in cervical cancer derived-cell lines Nehla Banu1,2, Annie Riera-Leal3, Jesse Haramati4, Pablo Cesar Ortiz-Lazareno5, Sandeep Surendra Panikar6, Blanca Estela Bastidas-Ramirez1, Gloria Yareli Gutierrez-Silerio1,2, Fabiola Solorzano-Ibarra1, Martha Cecilia Tellez-Bañuelos4, Jorge Gutierrez-Franco7, Miriam Ruth Bueno-Topete1, Ana Laura Pereira-Suarez2 and Susana del Toro-Arreola1,2*
Abstract Background: Although great progress has been made in treatment regimens, cervical cancer remains as one of the most common cancer in women worldwide. Studies focusing on molecules that regulate carcinogenesis may provide potential therapeutic strategies for cervical cancer. B7-H6, an activating immunoligand expressed by several tumor cells, is known to activate NK cell-mediated cytotoxicity once engaged with its natural receptor NKp30. However, the opposite, that is, the effects in the tumor cell triggered by B7-H6 after interacting with NKp30 has not yet been well explored. Methods: In this study, we evaluated the surface expression of B7-H6 by flow cytometry. Later, we stimulated B7H6 positive cervical cancer derived-cell lines (HeLa and SiHa) with recombinant soluble NKp30 (sNKp30) protein and evaluated biological effects using the impedance RTCA system for cell proliferation, the scratch method for cell migration, and flow cytometry for apoptosis. Cellular localization of B7-H6 was determined using confocal microscopy. (Continued on next page)
* Correspondence: [email protected] 1 Instituto de Enfermedades Crónico Degenerativas, Departamento de Biología Molecular y Genómica, CUCS, Universidad de Guadalajara, Sierra Mojada # 950, Colonia Independencia, CP, 44340 Guadalajara, Jalisco, Mexico 2 Laboratorio de Inmunología, Departamento de Fisiología, CUCS, Universidad de Guadalajara, Guadalajara, Mexico Full list of author information is available at the end of the article © The Author(s). 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applie
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