Bacterial Communities of Ballan Wrasse ( Labrus bergylta ) Eggs at a Commercial Marine Hatchery
- PDF / 2,240,959 Bytes
- 11 Pages / 595.276 x 790.866 pts Page_size
- 50 Downloads / 172 Views
Bacterial Communities of Ballan Wrasse (Labrus bergylta) Eggs at a Commercial Marine Hatchery Aileen Bone1 · Michaël Bekaert1 · Athina Papadopoulou1 · Stuart McMillan1 · Alexandra Adams1 · Andrew Davie1 · Andrew P. Desbois1 Received: 12 April 2020 / Accepted: 6 November 2020 © The Author(s) 2020
Abstract Ballan wrasse (Labrus bergylta, Ascanius 1767) are cleaner fish cultured in northern Europe to remove sea lice from farmed Atlantic salmon (Salmo salar, Linnaeus 1758). Despite increasing appreciation for the importance of the microbiota on the phenotypes of vertebrates including teleosts, the microbiota of wrasse eggs has yet to be described. Therefore, the aim of this present study was to describe the bacterial component of the microbiota of ballan wrasse eggs shortly after spawning and at 5 days, once the eggs had undergone a routine incubation protocol that included surface disinfection steps in a common holding tank. Triplicate egg samples were collected from each of three spawning tanks and analysis of 16S rRNA gene sequences revealed that 88.6% of reads could be identified to 186 taxonomic families. At Day 0, reads corresponding to members of the Vibrionaceae, Colwelliaceae and Rubritaleaceae families were detected at greatest relative abundances. Bacterial communities of eggs varied more greatly between tanks than between samples deriving from the same tank. At Day 5, there was a consistent reduction in 16S rRNA gene sequence richness across the tanks. Even though the eggs from the different tanks were incubated in a common holding tank, the bacterial communities of the eggs from the different tanks had diverged to become increasingly dissimilar. This suggests that the disinfection and incubation exerted differential effects of the microbiota of the eggs from each tank and that the influence of the tank water on the composition of the egg microbiota was lower than expected. This first comprehensive description of the ballan wrasse egg bacterial community is an initial step to understand the role and function of the microbiota on the phenotype of this fish. In future, mass DNA sequencing methods may be applied in hatcheries to screen for pathogens and as a tool to assess the health status of eggs.
Introduction Ballan wrasse (Labrus bergylta Ascanius 1767) are cleaner fish recognised as an established and important biological component of sea lice control in Atlantic salmon (Salmo salar Linnaeus, 1758) farming in northern Europe [1–3] The cleaner fish remove sea lice from infested salmon and recent efforts have reduced reliance on wild capture, with more Aileen Bone and Michaël Bekaert have contributed equally to this work. Electronic supplementary material The online version of this article (https://doi.org/10.1007/s00284-020-02286-8) contains supplementary material, which is available to authorized users. * Andrew P. Desbois [email protected] 1
Institute of Aquaculture, Faculty of Natural Sciences, University of Stirling, Stirling FK9 4LA, UK
than half of demand for cleaner fish now deli
Data Loading...
