Comparative analysis of microbial communities associated with bacteriomes, reproductive organs and eggs of the cicada Su
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ORIGINAL PAPER
Comparative analysis of microbial communities associated with bacteriomes, reproductive organs and eggs of the cicada Subpsaltria yangi Dandan Wang1 · Zhi Huang1 · Hong He2 · Cong Wei1
Received: 19 June 2017 / Revised: 9 September 2017 / Accepted: 16 September 2017 © Springer-Verlag GmbH Germany 2017
Abstract Plant sap-feeding insects of Hemiptera often form intimate symbioses with microbes to obtain nutrients. The cicada Subpsaltria yangi is the only species of the subfamily Tettigadinae known from China. Using highthroughput sequencing combined with fluorescence in situ hybridization analysis, we characterize the bacterial composition of the bacteriomes, testes, ovaries and eggs of two representative populations of this species which occur in different habitats and feed on different plant hosts. In both populations, the bacterial community diversity in the testes was significantly higher than that in other tissues. The obligate endosymbiont Candidatus Sulcia muelleri was observed in all samples and was dominant in the bacteriomes, ovaries and eggs. The usual co-resident endosymbiont Candidatus Hodgkinia cicadicola found in some other cicadas was not detected. Instead, a novel Rhizobiales bacterium which shows a ~ 81% 16S rDNA similarity to Ca. Hodgkinia cicadicola was detected. Given that the genome of Ca. Hodgkinia cicadicola exhibits rapid evolution, it is Communicated by Markus Nett. Electronic supplementary material The online version of this article (doi:10.1007/s00203-017-1432-8) contains supplementary material, which is available to authorized users. * Hong He [email protected] * Cong Wei [email protected] 1
Key Laboratory of Plant Protection Resources and Pest Management, Ministry of Education, College of Plant Protection, Northwest A&F University, Yangling, Shaanxi, People’s Republic of China
College of Forestry, Northwest A&F University, Yangling, Shaanxi, People’s Republic of China
2
possible that this novel Rhizobiales bacterium is a related endosymbiont with beneficial trophic functions similar to that of Ca. Hodgkinia cicadicola hosted by several certain other cicadas. The presence of the novel Rhizobiales species in other cicadas and its involvement with the adaptive evolution of related cicada hosts require further investigation. Discrepancy of bacterial communities associated with testes between the two populations may be closely related to the geographic isolation and divergence of habitats and host plants. Our results are informative for further studies of evolutionary divergence of related endosymbionts hosted in cicadas. Keywords Cicadidae · Endosymbiont · Candidatus Sulcia muelleri · Candidatus Hodgkinia cicadicola · Highthroughput sequencing · Fluorescence in situ hybridization
Introduction The relationship between endosymbionts and insects is particularly important in the order Hemiptera, which mainly feed on plant phloem or xylem sap (Moran et al. 2005). Plant sap contains rich carbohydrates, but is poor in nitrogenous compounds (Redak et al. 20
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