Bacteriological, cytological, and molecular investigation of Corynebacterium pseudotuberculosis , mycobacteria, and othe
- PDF / 322,596 Bytes
- 8 Pages / 595.276 x 790.866 pts Page_size
- 6 Downloads / 152 Views
VETERINARY MICROBIOLOGY - RESEARCH PAPER
Bacteriological, cytological, and molecular investigation of Corynebacterium pseudotuberculosis, mycobacteria, and other bacteria in caseous lymphadenitis and healthy lymph nodes of slaughtered sheep Thiago de Oliveira Zamprogna 1 & Dayana Ribeiro 2 & Vasco A.C. Azevedo 2 & Gustavo Henrique Batista Lara 1 & Rodrigo Garcia Motta 1 & Rodrigo Costa da Silva 3 & Amanda Keller Siqueira 1 & Geraldo de Nardi Júnior 4 & Fernando José Paganini Listoni 1 & Lorrayne de Souza Araújo Martins 1 & Aristeu Vieira da Silva 5 & Fábio Vinícius Ramos Portilho 1 & André da Rocha Mota 1 & Carolina Aparecida Rodrigues 1 & Beatriz Oliveira de Almeida 1 & Márcio Garcia Ribeiro 1 Received: 26 February 2020 / Accepted: 4 November 2020 # Sociedade Brasileira de Microbiologia 2020
Abstract Caseous lymphadenitis (CL) in sheep is a chronic contagious disease caused by Corynebacterium pseudotuberculosis, commonly characterized by abscess formation in peripheral lymph nodes and disseminated infections. Nonetheless, other microorganisms, including with zoonotic relevance, can be isolated from CL-resembling lymph nodes. Currently, mycobacteria have been reported in visceral granulomatous lesions in small ruminants, a fact that poses a public health issue, particularly in slaughtered sheep intended for human consumption. Cytology using fine needle aspiration and microbiological culturing are suitable tests for routine diagnostic, whereas present drawbacks and molecular methods have been confirmatory. Data about the occurrence of mycobacteria in both lymph nodes with aspect of CL and apparently healthy visceral nodes of sheep slaughtered for human consumption are scarce. In this study, 197 visceral lymph nodes of sheep showed lymphadenitis and 202 healthy visceral lymph nodes of slaughtered sheep intended for human consumption were submitted to conventional bacteriological diagnosis, mycobacteria culturing, and cytological evaluation. Compatible Corynebacterium isolates were subjected to multiplex PCR targeting 16S rRNA, rpoB, and pld genes to detect C. pseudotuberculosis. Based on microbiological identification, C. pseudotuberculosis (86/197; 43.7%), streptococci γ-hemolytic (17/197; 8.6%), and Trueperella pyogenes (12/197; 6.1%) were prevalent in lymph nodes with abscesses, as opposed to staphylococci (53/202; 26.2%) in apparently healthy lymph nodes. No mycobacteria were isolated. Cytology identified 49.2% (97/197) Gram-positive pleomorphic organisms (coryneform aspect). Multiplex PCR confirmed genetic material of C. pseudotuberculosis in 74.4% (64/86) of the samples with C. pseudotuberculosis isolation and 66% (64/97) samples with cytological coryneform aspect (κ = 86.78%; 95% CI = 79.87–93.68%). These findings emphasize the prevalence of C. pseudotuberculosis in abscess formation among peripheral lymph nodes of sheep. Other bacteria were also identified in lymph nodes sampled that resembling C. pseudotuberculosis–induced infections that may difficult the diagnosis. Multiplex PCR revealed a valuable assay
Data Loading...
