Molecular and cytological analysis of widely-used Gal4 driver lines for Drosophila neurobiology
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RESEARCH
Open Access
Molecular and cytological analysis of widely-used Gal4 driver lines for Drosophila neurobiology Anna A. Ogienko1†, Evgeniya N. Andreyeva1†, Evgeniya S. Omelina1, Anastasiya L. Oshchepkova1,2 and Alexey V. Pindyurin1,3* From 11th International Young Scientists School “Systems Biology and Bioinformatics” – SBB-2019 Novosibirsk, Russia. 24-28 June 2019
Abstract Background: The Drosophila central nervous system (CNS) is a convenient model system for the study of the molecular mechanisms of conserved neurobiological processes. The manipulation of gene activity in specific cell types and subtypes of the Drosophila CNS is frequently achieved by employing the binary Gal4/UAS system. However, many Gal4 driver lines available from the Bloomington Drosophila Stock Center (BDSC) and commonly used in Drosophila neurobiology are still not well characterized. Among these are three lines with Gal4 driven by the elav promoter (BDSC #8760, #8765, and #458), one line with Gal4 driven by the repo promoter (BDSC #7415), and the 69B-Gal4 line (BDSC #1774). For most of these lines, the exact insertion sites of the transgenes and the detailed expression patterns of Gal4 are not known. This study is aimed at filling these gaps. Results: We have mapped the genomic location of the Gal4-bearing P-elements carried by the BDSC lines #8760, #8765, #458, #7415, and #1774. In addition, for each of these lines, we have analyzed the Gal4-driven GFP expression pattern in the third instar larval CNS and eye-antennal imaginal discs. Localizations of the endogenous Elav and Repo proteins were used as markers of neuronal and glial cells, respectively. Conclusions: We provide a mini-atlas of the spatial activity of Gal4 drivers that are widely used for the expression of UAS–target genes in the Drosophila CNS. The data will be helpful for planning experiments with these drivers and for the correct interpretation of the results. Keywords: Drosophila, Gal4/UAS, CNS, Neurons, Glia, Driver, elav-Gal4, elavC155, Gal4repo, 69B-Gal4
* Correspondence: [email protected] † Anna A. Ogienko and Evgeniya N. Andreyeva contributed equally to this work. 1 Institute of Molecular and Cellular Biology, Siberian Branch of RAS, Novosibirsk 630090, Russia 3 Novosibirsk State University, Novosibirsk 630090, Russia Full list of author information is available at the end of the article © The Author(s). 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory
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