Beauveria bassiana ERL836 and JEF-007 with similar virulence show different gene expression when interacting with cuticl
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RESEARCH ARTICLE
Open Access
Beauveria bassiana ERL836 and JEF-007 with similar virulence show different gene expression when interacting with cuticles of western flower thrips, Frankniella occidentalis Sihyeon Kim1†, Jong Cheol Kim1†, Se Jin Lee2, Mi Rong Lee1, So Eun Park1, Dongwei Li1, Sehyeon Baek1, Tae Young Shin1 and Jae Su Kim1,3*
Abstract Background: Insect-killing fungal species, Beauveria bassiana, is as an environment-friendly pest management tool, and many isolates are on the track of industrialization. However, some of B. bassiana isolates show similar morphology and virulence against insect pests, and so it is hard to differentiate them. Herein we used two patented isolates, ERL836 and JEF-007, and investigated their virulence against western flower thrips, Frankliniella occidentalis, and further analyzed genome structures and transcriptional responses when interacting with cuticles of thrips to see possible differences on the initial step of fungal infection. Results: The two isolates showed no significant differences in fungal growth, conidial production, and virulence against thrips, and they were structurally similar in genome. But, in transcription level, ERL836 appeared to infect thrips easily, while JEF-007 appeared to have more difficulty. In the GO analysis of ERL836 DEGs (differentially expressed genes), the number of up-regulated genes was much larger than that of down-regulated genes, when compared to JEF-007 DEGs (more genes down-regulated). Interestingly, in the enrichment analysis using shared DEGs between two infecting isolates, plasma membrane-mediated transporter activity and fatty acid degradation pathway including cytochrome P450 were more active in infecting ERL836. Conclusion: The two B. bassiana isolates had similar morphology and virulence as well as genome structure, but in transcription level they differently interacted with the cuticle of western flower thrips. This comparative approach using shared DEG analysis could be easily applied to characterize the difference of the two B. bassiana isolates, JEF007 and ERL836. Keywords: Beauveria bassiana, Western flower thrips, Genome, Transcription, Cytochrome P450
* Correspondence: [email protected] † Sihyeon Kim and Jong Cheol Kim contributed equally to this work. 1 Department of Agricultural Biology, Jeonbuk National University, Jeonju 54596, South Korea 3 Department of Agricultural Convergence Technology, Jeonbuk National University, Jeonju 54596, South Korea Full list of author information is available at the end of the article © The Author(s). 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless in
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