Beneficial effects of Diplectria barbata (Wall. Ex C. B. Clarke) Franken et Roos extract on aging and antioxidants in vi
- PDF / 6,026,824 Bytes
- 13 Pages / 595.276 x 790.866 pts Page_size
- 66 Downloads / 138 Views
Toxicol Res. https://doi.org/10.1007/s43188-020-00064-z
Toxicological Research
ORIGINAL ARTICLE
Beneficial effects of Diplectria barbata (Wall. Ex C. B. Clarke) Franken et Roos extract on aging and antioxidants in vitro and in vivo Youngeun Hong1,2 · Hyunji Lee1,2 · Quangdon Tran1,2 · Choinyam Bayarmunkh3,4 · Damdindorj Boldbaatar3,4 · So Hee Kwon5 · Jongsun Park1,2 · Jisoo Park1,2,6 Received: 3 July 2020 / Revised: 21 August 2020 / Accepted: 1 September 2020 © Korean Society of Toxicology 2020
Abstract The purpose of this study is to explore the effects of Diplectria barbata (Wall. Ex C.B. Clarke) Franken & Roons (DFR) on wound healing, antioxidant and aging in Normal Human Dermal Fibroblast cell (NHDF) cells and mouse skin models. We investigated the effects of the aging process in vitro and in vivo. DFRtreated NHDF cells showed a concentration-dependent increase in the expression of extracellular matrix (ECM) proteins (Collagen-2.5-fold increase at 50 μg/ml, Elastin-1.5-fold increase at 1μg/ml) as well as an increase in proteins related to cell survival, differentiation, and development, while expression of aging proteins such as matrix metalloproteinase 3 (MMP-3) was decreased (5-fold decrease at 50 μg/ml). DFR treatment also led to enhanced expression of antioxidant proteins such as nuclear factor erythroid 2-related factor 2 (10-fold increase at 50 μg/ml) and heme oxygenase 1 (1.5-fold increase at 25 μg/ml). To further investigate the antioxidative effects of DFR extracts, the 2,2-diphenyl-1-picrylhydrazyl radical scavenging activities were also evaluated. DFR extracts improved wound healing and resulted in increased expression of ECM proteins, while enzymes involved in collagen degradation, including MMP-3, were decreased in NHDF cells as well as in a mouse model. This study demonstrates the anti-aging, antioxidant, and wound healing properties of DFR extracts. Therefore, DFR extracts present may facilitate skin protection and care. Keywords Anti-aging · Anti-oxidant · Wound healing · Diplectria barbata (Wall. Ex C.B. Clarke) Franken & Roos · Extracts · NHDF cells Youngeun Hong and Hyunji Lee equally have contributed to this work. * Jongsun Park [email protected] * Jisoo Park [email protected] 1
Abbreviations ARE Antioxidant response element DFR Diplectria barbata (Wall. Ex C.B. Clarke) Franken & Roos DPPH 2,2-Diphenyl-1-picryl-hydrazyl-hydrate ECM Extracellular matrix HO-1 Heme oxygenase 1 MMP-3 Matrix metalloproteinase 3 MEK Mitogen-activated protein kinase kinase NHDF Normal human dermal fibroblast NRF2 Nuclear factor erythroid 2-related factor 2 PKB Protein kinase B ROS Reactive oxygen species TAC Total antioxidant capacity
Department of Pharmacology, Metabolic Syndrome and Cell Signaling Laboratory, Institute for Cancer Research, College of Medicine, Chungnam National University, Daejeon 35015, South Korea
2
Department of Medical Science, College of Medicine, Chungnam National University, Daejeon 35015, South Korea
3
Department of Graduate Education, Graduate school, Mongol
Data Loading...
