Bio-inspired synthesis of superparamagnetic iron oxide nanoparticles for enhanced in vitro anticancer therapy
- PDF / 768,842 Bytes
- 6 Pages / 612 x 792 pts (letter) Page_size
- 93 Downloads / 258 Views
Research Letter
Bio-inspired synthesis of superparamagnetic iron oxide nanoparticles for enhanced in vitro anticancer therapy Thangavel Shanmugasundaram, Actinobacterial Research Laboratory, Department of Microbiology, Periyar University, Periyar Palkalai Nagar, Salem 636 011, Tamil Nadu, India; DRDO-BU Centre for Life Sciences, Bharathiar University Campus, Coimbatore 641 046, Tamil Nadu, India Manikkam Radhakrishnan, Centre for Drug Discovery and Development, Sathyabama Institute of Science and Technology (Deemed to be University), Jeppiaar Nagar, Chennai 600 119, Tamil Nadu, India Arasu Poongodi, Department of Biochemistry, Sri Ramachandra University, Chennai 600 116, Tamil Nadu, India Krishna Kadirvelu, DRDO-BU Centre for Life Sciences, Bharathiar University Campus, Coimbatore 641 046, Tamil Nadu, India Ramasamy Balagurunathan, Actinobacterial Research Laboratory, Department of Microbiology, Periyar University, Periyar Palkalai Nagar, Salem 636 011, Tamil Nadu, India Address all correspondence to Ramasamy Balagurunathan at [email protected] (Received 29 January 2018; accepted 27 February 2018)
Abstract Superparamagnetic iron oxide nanoparticles (SPIONPs) are successfully synthesized in this study by co-precipitation method using actinobacterial metabolites as reducing agent. Physicochemical and morphological features of the nanoparticles (NPs) are analyzed by Fourier-transform infrared spectroscopy, x-ray-based techniques, vibrating sample magnetometer, thermal gravimetric analysis, and electron microscopic analysis, with an average size of 15–30 nm. Anticancer activity of the magnetite-NPs is systematically evaluated on HeLa cells using MTT assay, Hoechst nuclear staining, acridine orange/ethidium bromide dual staining and flow cytometric analysis. The obtained results open a new route for biosynthesis of SPIONPs, which to be used for various biomedical applications, particularly in cancer therapy.
Introduction Magnetic nanoparticles (NPs), particularly superparamagnetic iron oxide nanoparticles (SPIONPs) with appropriate surface chemistry have emerged as a promising new platform in biomedical and bioengineering applications, such as protein and enzyme immobilization, tissue repair, immunoassay, magnetic resonance imaging, hyperthermia, hybridization and detection of DNA, drug delivery, etc.[1–3] Modern applications require that these NPs have high magnetization values and size in nano regime with overall narrow particle size distribution so that the particles have identical physicochemical properties.[1] Magnetite is the most stable among iron oxide (IO; Fe3O4) with a high resistance to corrosion, superparamagnetic, high coercivity, high magnetic susceptibility, low cost, and low Curie temperature.[4] Currently, biomolecules-mediated synthesis of IONPs has been considered a more environmentally sound, non-toxic, biocompatible, and more cost-effective alternative to chemical and physical methods of synthesis.[5] With this view, the present study focuses the actinobacterial metabolites-mediated synthesi
Data Loading...
