Biodegradation of natural rubber and deproteinized natural rubber by enrichment bacterial consortia
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ORIGINAL PAPER
Biodegradation of natural rubber and deproteinized natural rubber by enrichment bacterial consortia Lan Huong Nguyen . Hoang Dung Nguyen . P. Thao Tran . Thi Thuong Nghiem . Thi Thanh Nguyen . Viet Linh Dao . Trung Nghia Phan . Anh Kim To . Masashi Hatamoto . Takashi Yamaguchi . Daisuke Kasai . Masao Fukuda
Received: 9 June 2020 / Accepted: 5 September 2020 Ó Springer Nature B.V. 2020
Abstract This study examined the biodegradation of natural rubber (NR) and deproteinized natural rubber (DPNR) by bacterial consortia enriched from a rubberprocessing factory’s waste in Vietnam. The results reveal the degradation in both NR and DPNR, and the DPNR was degraded easier than NR. The highest weight loss of 48.37% was obtained in the fourth enrichment consortium with DPNR, while 35.39% was obtained in the fifth enrichment consortium with NR after 14 days of incubation. Nitrogen content and fatty acid content determined by Kjeldahl method and
L. H. Nguyen (&) H. D. Nguyen T. T. Nguyen V. L. Dao A. K. To School of Biotechnology and Food Technology, Hanoi University of Science and Technology, No 1, Dai Co Viet street, Hanoi, Vietnam e-mail: [email protected]
fourier transform infrared spectroscopy (FTIR), respectively, were decreased significantly after being incubated with the consortia. Structure of degraded rubber film analyzed by nuclear magnetic resonance spectroscopy showed the presence of aldehyde group, a sign of rubber degradation. Bacterial cells tightly adhering and embedding into NR and DPNR films were observed by scanning electron microscopy. There were differences in the bacterial composition of the consortia with NR and DPNR, which were determined by metagenomic analysis using 16S rRNA gene sequencing. The phyla Bacteroidetes and Proteobacteria may play a role in the degradation of nonisoprene compounds such as protein or lipid, while the phylum Actinobacteria plays a crucial role in the degradation of rubber hydrocarbon in all consortia. Keywords Natural rubber Deproteinized natural rubber Biodegradation Bacterial consortium
P. T. Tran M. Hatamoto T. Yamaguchi Department of Science of Technology Innovation, Nagaoka University of Technology, Nagaoka, Japan T. T. Nghiem T. N. Phan School of Chemical Engineering, Hanoi University of Science and Technology, Hanoi, Vietnam V. L. Dao D. Kasai M. Fukuda Department of Bioengineering, Nagaoka University of Technology, Nagaoka, Japan M. Fukuda Department of Biological Chemistry, Chubu University, Kasugai, Japan
Introduction Natural rubber (NR) is an important polymer with useful properties, such as elasticity, extensibility, resistance to tearing and abrasion; therefore, it has been widely applied in the production of tires, latex gloves, medical products and toys (Greve 2000). Fresh NR latex contains 25–35% rubber by weight, which is dispersed as rubber particles in water with some minor
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Biodegradation
non-rubber constituents such as proteins, phospholipids, gl
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