Biofunctionalization of porcine-derived collagen matrices with platelet rich fibrin: influence on angiogenesis in vitro
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ORIGINAL ARTICLE
Biofunctionalization of porcine-derived collagen matrices with platelet rich fibrin: influence on angiogenesis in vitro and in vivo Sebastian Blatt 1,2 & Valentin Burkhardt 1 & Peer W. Kämmerer 1 & Andreas M. Pabst 3 & Keyvan Sagheb 1 & Martin Heller 4 & Bilal Al-Nawas 1 & Eik Schiegnitz 1 Received: 25 September 2019 / Accepted: 15 January 2020 # The Author(s) 2020
Abstract Objectives Porcine-derived collagen matrices (CM) can be used for oral tissue regeneration, but sufficient revascularization is crucial. The aim of this study was to analyze the influence of platelet-rich fibrin (PRF) on angiogenesis of different CM in vitro and in vivo. Materials and methods Three different CM (mucoderm®, jason®, collprotect®) were combined with PRF in a plotting process. Growth factor release (VEGF, TGF-β) was measured in vitro via ELISA quantification after 1,4 and 7 days in comparison to PRF alone. In ovo yolk sac (YSM) and chorion allantois membrane (CAM) model, angiogenic potential were analyzed in vivo with light- and intravital fluorescence microscopy after 24 h, then verified with immunohistochemical staining for CD105 and αSMA. Results Highest growth factor release was seen after 24 h for all three activated membranes in comparison to the native CM (VEGF 24 h: each p < 0.05; TGF-β: each p < 0.001) and the PRF (no significant difference). All activated membranes revealed a significantly increased angiogenic potential in vivo after 24 h (vessels per mm2: each p < 0.05; branching points per mm2: each p < 0.01; vessel density: each p < 0.05) and with immunohistochemical staining for CD105 (each p < 0.01) and αSMA (each p < 0.05). Conclusions PRF improved the angiogenesis of CM in vitro and in vivo. Clinical relevance Bio-functionalization of CM with PRF could easily implemented in the clinical pathway and may lead to advanced soft tissue healing. Keywords Angiogenesis . Vascularization . Collagen matrices . Platelet-rich fibrin . Tissue engineering
Introduction Porcine-derived collagen matrices (CM) are widely used in oral surgery, periodontology and implantology for hard and Electronic supplementary material The online version of this article (https://doi.org/10.1007/s00784-020-03213-8) contains supplementary material, which is available to authorized users. * Sebastian Blatt [email protected] 1
Department of Oral- and Maxillofacial Surgery, University Medical Center, Augustusplatz 2, 55131 Mainz, Germany
2
Platform for Biomaterial Research, Biomatics Group, University Medical Center, Langenbeckstrasse 1, 55131 Mainz, Germany
3
Department of Oral- and Maxillofacial Surgery, Federal Armed Forces Hospital, Rübenacher Str. 170, 56072 Koblenz, Germany
4
Department of Gynecology, University Medical Center, Langenbeckstrasse 1, 55131 Mainz, Germany
soft tissue regeneration [1]. Principally, there are two different indications in the clinical routine: on the one hand, CM can be used as soft tissue grafts as a possible alternative to autogenously connective tissue [2]. Derived from po
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