Biogenesis of silver nanoparticles using leaf extracts of Asparagus racemosus and Sophora interrupta : structure charact
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Biogenesis of silver nanoparticles using leaf extracts of Asparagus racemosus and Sophora interrupta: structure characterization, antibacterial and anticancer studies Bethu Murali Satyanarayana1 · Netala Vasudeva Reddy2 · Siva kesava Rao Kommula3 · J. Venkateswara Rao1 Received: 12 May 2020 / Accepted: 13 October 2020 © Springer Nature Switzerland AG 2020
Abstract The present investigation reports simple and eco-friendly biogenesis of silver nanoparticles (AgNPs) using leaf extracts of Asparagus racemosus and Sophora interrupta. UV–Vis spectra showed absorption peaks at 413 and 420 nm respectively corresponding to A. racemosus (AR-AgNPs) and S. interrupta (SI-AgNPs). TEM, DLS, XRD and TGA studies revealed that biogenic AgNPs are monodispersed, spherical shaped, thermally stable, FCC crystals and 4–15 nm in size. Zeta potential values were found to be − 28.9 and − 18.6 mV respectively corresponding to SI-AgNPs and AR-AgNPs. SI-AgNPs exhibited strong antibacterial activities against Escherichia coli, Klebsiella pneumoniae, Bacillus subtilis and Micrococcus luteus with inhibition zones of 11.8, 10.5, 9.8 and 9.1 mm respectively. Further SI-AgNPs exhibited significant cytotoxic properties against SKOV3, DU149, PC3 and A549 cells with IC50 values of 120.87, 191.34, 235.06 and 314.27 µg/mL respectively. SIAgNPs induce pronounced apoptotic effects on SKOV3 cells including loss of membrane integrity, chromatin condensation (pyknosis) and nuclear fragmentation (karyorrhexis). SI-AgNPs exhibited significant cell migration inhibition by reducing the wound healing of SKOV3 monolayers. Phytosynthesized AgNPs were found to be biocompatible towards normal (CHO) cells.
Bethu Murali Sathyanarayana and Netala Vasudeva Reddy contributed equally to this work. * Bethu Murali Satyanarayana, [email protected] | 1Applied Biology Division, CSIR-Indian Institute of Chemical Technology, Hyderabad, Telangana 500007, India. 2Department of Biotechnology, Sri Venkateswara University, Tirupati, Andhra Pradesh 517502, India. 3Department of Biochemistry, Dr. Y.S.R. Horticultural University, Venkataramanna Gudem, Andhra Pradesh, India. SN Applied Sciences
(2020) 2:1857
| https://doi.org/10.1007/s42452-020-03699-7
Vol.:(0123456789)
Research Article
SN Applied Sciences
(2020) 2:1857
| https://doi.org/10.1007/s42452-020-03699-7
Graphic abstract
Keywords Silver nanoparticles · Sophora interrupta · Antibacterial activity · Anticancer activity · Apoptotic studies · Wound healing assay
1 Introduction Nanotechnology has become one of the most emerging areas that intertwine all the fields of science including physics, chemistry, biology, medicine and material science [1]. Nanobiotechnology involves the eco-friendly synthesis and biomedical applications of different types of metal and metal oxide nanomaterials [2]. Metallic nanomaterials, particularly silver nanoparticles (AgNPs) have attracted global attention due to their unique physico-chemical and biological properties including optical, electronic, thermal, optoelectronic, catalytic,
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