Rapid Green Synthesis of Biogenic Silver Nanoparticles Using Cinnamomum tamala Leaf Extract and its Potential Antimicrob
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Rapid Green Synthesis of Biogenic Silver Nanoparticles Using Cinnamomum tamala Leaf Extract and its Potential Antimicrobial Application Against Clinically Isolated Multidrug-Resistant Bacterial Strains Shib Shankar Dash 1 & Sovan Samanta 2 & Sananda Dey 2 & Biplab Giri 2 & Sandeep Kumar Dash 2 Received: 14 November 2019 / Accepted: 3 March 2020 # Springer Science+Business Media, LLC, part of Springer Nature 2020
Abstract Cinnamomum tamala is Indian bay leaves also known as Tej patta commonly used in the preparation of delicious food for its sweet aroma and tremendous medicinal values. In this study, the significant concentration-dependent free radical scavenging and antioxidant efficacy of the aqueous extracts of bay leaves has been determined using DPPH (2, 2-diphenyl-l-picrylhydrazyl) radical scavenging, ferric ion-reducing power assay, and hydrogen peroxide radical scavenging assay. The leaf extract has also been utilized in the rapid synthesis of silver nanoparticles (AgNPs) under mild conditions (30 min reaction time at 70 °C) without the addition of extra stabilizing or capping agents. Mostly spherical shaped particles were formed with diameter ranging from 10 to 12 nm as evident by HRTEM imaging. The silver nanoparticles were also characterized using FTIR, XRD, and UV-visible spectroscopic techniques. The antibacterial effect of the synthesized AgNPs was studied against three clinically isolated multidrug-resistant bacterial strains (Escherichia coli (EC-1), Klebsiella pneumonia (KP-1), and Staphylococcus aureus (SA1)). The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of AgNPs against EC-1 were 12.5 and 15 μg/mL and in SA-1 were 10 and 50 μg/mL, and in the case of KP-1, both values were 12.5 μg/mL. It was also noted that 8 h treatment duration using AgNPs was sufficient to eliminate all types of bacterial growth as evidenced by time-dependent killing kinetic assays. The biocompatibilities of AgNPs were also tested against human health RBCs, and it was observed that it did not show any significant toxicity up to 50 μg/mL concentration. Keywords Cinnamomum tamala . Antioxidant . Antibacterial . Silver nanoparticles . Green synthesis
Introduction Emergence of drug resistance in microbial pathogens has been documented as a key health threat in the community. Antibiotics are the best prescribed treatment policy against
Electronic supplementary material The online version of this article (https://doi.org/10.1007/s12011-020-02107-w) contains supplementary material, which is available to authorized users. * Shib Shankar Dash [email protected] 1
Department of Chemistry, Government General Degree College, Salboni , Paschim Medinipur, West Bengal 721516, India
2
Department of Physiology, University of Gour Banga, Malda, West Bengal 732103, India
the bacterial exposure due to satisfactory outcomes. But, inappropriate prescription policies, lack of knowledge, and non availability of new class’s antibiotics create a major problem which is commonly known as antibiotic resist
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