Investigating Physicochemical and Biological Properties of Chemical Silver Nanoparticles and Green Silver Nanoparticles
- PDF / 1,636,947 Bytes
- 11 Pages / 595.276 x 790.866 pts Page_size
- 24 Downloads / 246 Views
(0123456789().,-volV)(0123456789(). ,- volV)
ORIGINAL PAPER
Investigating Physicochemical and Biological Properties of Chemical Silver Nanoparticles and Green Silver Nanoparticles Mediated with Morus alba L. Extracts and the Related Antimicrobial Mechanism Chunli Wang1 • Yanan Jia1 • Zhongqin Chen1 • Ruilin Li1 • Nannan Li1 • Qirou Wang1 • Min Zhang2,3 Haixia Chen1
•
Received: 10 January 2020 / Accepted: 3 November 2020 Springer Science+Business Media, LLC, part of Springer Nature 2020
Abstract In order to investigate the physicochemical properties and bioactivities of AgNPs prepared using green and chemical approaches and further explore the related antibacterial mechanism, one kind of chemical AgNPs (C-AgNPs) and three kinds of green AgNPs (G-AgNPs) mediated with aqueous extracts of Morus alba L. were prepared and characterized. All G-AgNPs displayed higher antioxidant activities in DPPH radical scavenging, reducing power and total antioxidant assays than C-AgNPs and extracts. Meanwhile, all G-AgNPs exerted significantly (P \ 0.05) enhanced antimicrobial activities against various pathogens compared with C-AgNPs and extracts. Among prepared AgNPs, ML-AgNPs mediated with aqueous extract of Morus alba L. leaves exhibited the most powerful antibacterial effect with the largest inhibition zones (13.11 ± 0.76 mm for S. aureus, 13.27 ± 0.22 mm for E. coli) and the lowest minimum inhibition concentrations against S. aureus (6.125 lg/ml) and E. coli (3.125 lg/ml). ML-AgNPs could inhibit the growths of S. aureus and E. coli in timeand dose-dependent modes, and even could disrupt the bacterial structure integrality resulting in the leakage of intracellular contents. G-AgNPs, especially ML-AgNPs, with remarkable bioactivities could replace C-AgNPs as related agents applied in agriculture and medicine. Keywords Morus alba L. Green synthesis Silver nanoparticles Antioxidant Antibacterial mechanism
Introduction
Electronic supplementary material The online version of this article (https://doi.org/10.1007/s10876-020-01932-2) contains supplementary material, which is available to authorized users. & Haixia Chen [email protected] 1
Tianjin Key Laboratory for Modern Drug Delivery & HighEfficiency, School of Pharmaceutical Science and Technology, Tianjin University, Tianjin 300072, People’s Republic of China
2
Tianjin Agricultural University, Tianjin 300384, People’s Republic of China
3
State Key Laboratory of Nutrition and Safety, Tianjin University of Science & Technology, Tianjin 300457, People’s Republic of China
As one of interesting subjects in the field of nanotechnology, metal nanoparticles (MNPs) exert specific physical, chemical and biological properties owing to large surface to volume ratio compared to their bulk counterparts [1, 2]. As a kind of well-known MNPs, silver nanoparticles (AgNPs), always attract extensive attentions due to their considerably biomedical and pharmaceutical potentials as larvicidal, antioxidant, antibacterial and anticancer agents [3, 4]. AgNPs were traditionally fabricate
Data Loading...
