Biomimetic angle-ply multi-lamellar scaffold for annulus fibrosus tissue engineering
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TISSUE ENGINEERING CONSTRUCTS AND CELL SUBSTRATES Original Research
Biomimetic angle-ply multi-lamellar scaffold for annulus fibrosus tissue engineering Tongxing Zhang1,2 Lilong Du1,2 Jianing Zhao1 Ji Ding1,2 Peng Zhang1 Lianyong Wang3 Baoshan Xu1,2 ●
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Received: 29 December 2019 / Revised: 21 May 2020 / Accepted: 12 July 2020 © Springer Science+Business Media, LLC, part of Springer Nature 2020
Abstract Constructing a biomimetic scaffold that replicates the complex architecture of intervertebral disc annulus fibrosus (AF) remains a major goal in AF tissue engineering. In this study, a biomimetic angle-ply multi-lamellar polycaprolactone/silk fibroin (PCL/SF) AF scaffold was fabricated. Wet-spinning was used to obtain aligned PCL/SF microfiber sheets, and these were excised into strips with microfibers aligned at +30° or −30° relative to the strip long axis. This was followed by stacking two strips with opposing fiber alignment and wrapping them concentrically around a mandrel. Our results demonstrated that the scaffold possessed spatial structure and mechanical properties comparable to natural AF. The scaffold supported rabbit AF cells adhesion, proliferation, infiltration and guided oriented growth and extracellular matrix deposition. In conclusion, our angle-ply multi-lamellar scaffold offers a potential solution for AF replacement therapy and warrants further attention in future investigations. Graphical Abstract
1 Introduction
These authors contributed equally: Tongxing Zhang, Lilong Du, Jianing Zhao * Baoshan Xu [email protected] 1
Tianjin Medical University, Tianjin, PR China
2
Department of Spine Surgery, Tianjin Hospital, Tianjin, PR China
3
The Key Laboratory of Bioactive Materials, Ministry of Education, College of Life Sciences, Nankai University, Tianjin 300071, PR China
Intervertebral disc (IVD) degeneration often causes chronic pain and disability, leading to a substantial global socioeconomic burden [1, 2]. Current treatments for IVD degeneration focus on conservative approaches or surgical procedures such as spinal fusion or IVD replacement [3–5]. However, these strategies are only effective in relieving symptomatic pain without restoring biological function of IVD. The field of tissue engineering and the use of biomaterial-based approaches can offer biological strategies to help rescue IVD degeneration.
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Journal of Materials Science: Materials in Medicine (2020)31:67
Fig. 1 Schematic of engineering of angle-ply multi-lamellar AFCsscaffold construction. a Wet spinning process; b Circumferentially oriented microfiber tube; c–f Sheets were excised into strips with
microfibers alignment at +30°/−30°; g, h Seeding AFCs onto +30°/ −30° strips; i Two strips with opposing fiber orientation were stacked and wrapped concentrically around stainless-steel rod
The annulus fibrosus (AF), situated at the radial periphery of IVD, is composed of concentric collagen lamellae, which contains highly oriented collagen fibers tilted 30° from the horizont
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