Biosensors Based on Sandwich Assays

This book shows the various sandwich assays that are constructed from recognition molecules, such as antibodies, oligonucleotide sequences and aptamers, developed as a result of nano- and biotechnology advances. It consists of ten chapters presenting inte

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Biosensors Based on Sandwich Assays

Biosensors Based on Sandwich Assays

Fan Xia Xiaojin Zhang Xiaoding Lou Quan Yuan •



Editors

Biosensors Based on Sandwich Assays

123

Editors Fan Xia Faculty of Materials Science and Chemistry China University of Geosciences Wuhan, Hubei China Xiaojin Zhang Faculty of Materials Science and Chemistry China University of Geosciences Wuhan, Hubei China

Xiaoding Lou Faculty of Materials Science and Chemistry China University of Geosciences Wuhan, Hubei China Quan Yuan College of Chemistry and Molecular Sciences Wuhan University Wuhan, Hubei China

ISBN 978-981-10-7834-7 ISBN 978-981-10-7835-4 https://doi.org/10.1007/978-981-10-7835-4

(eBook)

Library of Congress Control Number: 2017962984 © Springer Nature Singapore Pte Ltd. 2018 This work is subject to copyright. All rights are reserved by the Publisher, whether the whole or part of the material is concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfilms or in any other physical way, and transmission or information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed. The use of general descriptive names, registered names, trademarks, service marks, etc. in this publication does not imply, even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. The publisher, the authors and the editors are safe to assume that the advice and information in this book are believed to be true and accurate at the date of publication. Neither the publisher nor the authors or the editors give a warranty, express or implied, with respect to the material contained herein or for any errors or omissions that may have been made. The publisher remains neutral with regard to jurisdictional claims in published maps and institutional affiliations. Printed on acid-free paper This Springer imprint is published by Springer Nature The registered company is Springer Nature Singapore Pte Ltd. The registered company address is: 152 Beach Road, #21-01/04 Gateway East, Singapore 189721, Singapore

Contents

1

Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Xiaojin Zhang and Fan Xia

1

2

Colorimetric Sandwich Assays for Protein Detection . . . . . . . . . . . Xiaoqing Yi, Rui Liu, Xiaoding Lou and Fan Xia

15

3

Fluorescence Sandwich Assays for Protein Detection . . . . . . . . . . . Fujian Huang and Fan Xia

29

4

Electrochemical Sandwich Assays for Protein Detection . . . . . . . . . Hui Li, Shaoguang Li and Fan Xia

47

5

Sandwich Assays Based on SPR, SERS, GMR, QCM, Microcantilever, SAW, and RRS Techniques for Protein Detection . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Shenshan Zhan, Xiaoding Lou, Pei Zhou and Fan Xia

69 93

6

Colorimetric Sandwich Assays for Nucleic Acid Detection . . . . . . . Xiaoxia Hu and Q