Carotenoid Production by Red Yeast Isolates Grown in Agricultural and "Mandi" Waste
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ORIGINAL PAPER
Carotenoid Production by Red Yeast Isolates Grown in Agricultural and "Mandi" Waste Sweta Sinha1 · Gunjan Singh1 · Anju Arora2 · Debarati Paul1 Received: 12 May 2020 / Accepted: 26 October 2020 © Springer Nature B.V. 2020
Abstract Purpose Microbial synthesis of carotenoids has gained interest as an alternative to synthetic carotenoid production due to its easy extraction and high yield. The current study investigates the usability of fruit and vegetable waste- ‘mandi’ waste and agricultural waste as growth medium for carotenoid production by different isolated yeast strains. Methods In this study, five isolated yeast strains were identified which were able to utilize C5 and C6 sugars. These yeast strains were compared for their carotenoid production and growth properties when cultivated in inexpensive growth media such as, vegetable ‘mandi’-waste and paddy straw hydrolysate (agricultural waste). During the cultivation, optical density (600 nm), cell weight (wet biomass and dry biomass), total sugar and reducing sugar in selected culture media were determined at different time intervals. The microbial carotenoids were extracted in DMSO (Dimethyl sulfoxide) and were finally transferred to hexane for separation by liquid–liquid extraction. Results Using ‘mandi’-waste hydrolysate (waste extract), the strains gave best results with biomass ranging from 29 ± 0.18 to 54 ± 0.14 g/L and the maximum carotenoid produced ranged between 38 ± 0.8 and 62 ± 0.93 mg/L. Conclusion Based on our results, ‘Mandi’ waste hydrolysate was found to be the most potent source for carotenoid production. This report provides a suitable bioprocess for the production of yeast-carotenoids, using vegetable ‘mandi’ waste (waste extract) and agricultural waste hydrolysates (paddy straw hydrolysate) via sustainable and cost-effective strategy that may be scaled up in future.
Electronic supplementary material The online version of this article (https://doi.org/10.1007/s12649-020-01288-8) contains supplementary material, which is available to authorized users. * Debarati Paul [email protected] 1
Amity Institute of Biotechnology, Amity University, Uttar Pradesh (AUUP), Noida 201313, India
ICAR-Indian Agricultural Research Institute, New Delhi 110012, India
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Waste and Biomass Valorization
Graphic Abstract
Keywords Isolation · Phylogeny · Biomass · Pigments · Waste hydrolysate Abbreviations WE Waste extract MM Minimal medium PSH Paddy straw hydrolysate HPLC High performance liquid chromatography OD Optical density DMSO Dimethyl sulpho-oxide DCW Dry cell weight YPD Yeast extract, peptone, dextrose RT Room temperature
Statement of Novelty The novelty of this work is that it provides a suitable bioprocess for the production of yeast-carotenoids, using vegetable ‘mandi’ waste (waste extract) and agricultural waste hydrolysates (paddy straw hydrolysate) via sustainable and
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cost-effective strategy that may be scaled up in future. Paddy straws are being burnt by farmers causing major
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