Cell-free supernatant of Streptococcus salivarius M18 impairs the pathogenic properties of Pseudomonas aeruginosa and Kl
- PDF / 3,572,412 Bytes
- 16 Pages / 595.276 x 790.866 pts Page_size
- 15 Downloads / 167 Views
ORIGINAL PAPER
Cell‑free supernatant of Streptococcus salivarius M18 impairs the pathogenic properties of Pseudomonas aeruginosa and Klebsiella pneumonia Sinem Tunçer1,2 · Sevinç Karaçam2,3 Received: 24 April 2020 / Revised: 15 July 2020 / Accepted: 24 July 2020 © Springer-Verlag GmbH Germany, part of Springer Nature 2020
Abstract M18 strain of Streptococcus salivarius is a bacterial replacement probiotic that has been suggested for use in the oral cavity. Here, we have shown that S. salivarius M18 cell-free supernatant reduced the growth of the two most common human pathogens Pseudomonas aeruginosa and Klebsiella pneumonia and sensitized the pathogenic bacteria to antibiotic. Besides, the supernatant inhibited biofilm formation of P. aeruginosa drastically. For pinpointing the biomolecular changes that occurred in P. aeruginosa incubated with the probiotic supernatant, attenuated total reflectance-Fourier transform infrared (ATR-FTIR) spectroscopy was used. Unsupervised learning algorithms, principal component analysis (PCA) and hierarchical cluster analysis (HCA), and intensity analyses of individual spectral bands exhibited comprehensive alterations in the polysaccharide and lipid contents and compositions of P. aeruginosa cultivated with S. salivarius M18 cell-free supernatant. These results indicate that S. salivarius M18 has the potential for the prevention or alleviation of different pathogen-induced infections along with the infections of oral pathogens. Keywords Streptococcus salivarius M18 · Pseudomonas aeruginosa · Klebsiella pneumonia · Biofilm · Polysaccharide · Lipid
Introduction The World Health Organization describes probiotics as “Live microorganisms which, when administered in adequate amounts, confer a health benefit to the host.” Probiotic microorganisms have been shown to help in the treatment of
Communicated by Erko Stackebrandt. Electronic supplementary material The online version of this article (https://doi.org/10.1007/s00203-020-02005-8) contains supplementary material, which is available to authorized users. * Sinem Tunçer [email protected] 1
Vocational School of Health Services, Department of Medical Services and Techniques, Bilecik Şeyh Edebali University, 11230 Bilecik, Turkey
2
Biotechnology Application and Research Center, Bilecik Şeyh Edebali University, 11230 Bilecik, Turkey
3
Department of Biotechnology, Bilecik Şeyh Edebali University, 11230 Bilecik, Turkey
dental caries, pathologies of the gastrointestinal tract, acute respiratory tract infections as well as multiple autoimmune conditions and inflammatory disorders (Liu et al. 2018a, b; Hasslöf and Stecksén-Blicks 2019; Sengupta and Paramasivan 2019). Furthermore, in vivo studies and in vitro models suggested a supportive role of probiotics in the management of complicated burn injury, e.g., of burn wounds infected with Pseudomonas aeruginosa (Argenta et al. 2016; Satish et al. 2017; Besser et al. 2019). The observed antipathogenic effects of probiotic bacteria rely on a variety of competitiv
Data Loading...
