Biotechnological production and statistical optimization of fungal xylanase by bioconversion of the lignocellulosic biom
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ORIGINAL ARTICLE
Biotechnological production and statistical optimization of fungal xylanase by bioconversion of the lignocellulosic biomass residues in solid-state fermentation Zahra Azzouz 1
&
Azzeddine Bettache 1 & Ibtissem Djinni 1 & Nawel Boucherba 1 & Said Benallaoua 1
Received: 6 July 2020 / Revised: 2 September 2020 / Accepted: 12 September 2020 # Springer-Verlag GmbH Germany, part of Springer Nature 2020
Abstract Most of the lignocellulosic biomass residues are decomposed and transformed by a variety of microbes in the natural environment. The xylanase production from Aspergillus niger strain BG has been produced using wheat bran under solid-state fermentation (SSF). One factor at a time approach (OFAT) was used to optimize the effect of the incubation period, initial pH, moisture content, and cultivation temperature on the xylanase production. Furthermore, experiments were designed with a Box–Behnken design (BBD) on the same variables using response surface methodology (RSM). Analysis of variance (ANOVA) was carried out and the xylanase production was expressed with a mathematical equation depending on the factors. Maximum xylanase yield after OFAT approach and RSM optimization was significant with maximum values of 4008.25 ± 3.73 U/g of dry substrate (U/gds) and 5427.51 ± 4.4 U/gds which have been recorded respectively compared with the initial conditions (1899.02 ± 1.6 U/gds) after 7 days of fermentation. The effects of individual, interaction, and square terms on xylanase production were represented using the non-linear regression equations with significant R2 and p values. The optimum conditions established by RSM method for the maximum xylanase production were obtained with a pH media of 2.5 at 37 °C using wheat bran as 84% humidified substrate after 66 h of incubation, this conditions resulted in 65.01% increased level of the xylanase production than produced in the initial conditions. Xylanase production from Aspergillus niger strain BG using RSM is considered advantageous for bioconversion of the agriculture residues.
Keywords Lignocellulosic biomass residues . Optimization . Xylanase . Aspergillus niger strain BG . Solid-state fermentation Abbreviations OFAT One factor at a time BBD Box–Behnken design RSM Response surface methodology Highlights • Optimization of the xylanase production by wheat bran biodegradation on SSF. • Xylanase production was enhanced at the different conditions using OFAT and RSM approaches. • RSM is underline with optimum OFAT conditions to select the optimal range. • The xylanase production has been enhanced to 26.15% using RSM. * Zahra Azzouz [email protected] * Said Benallaoua [email protected] 1
Laboratoire de Microbiologie Appliquée (LMA), Faculté des Sciences de la Nature et de la Vie, Université de Bejaia, 06000 Bejaia, Algeria
ANOVA U/gds SSF PDA
Analysis of variance Unit per gram of dry substrate Solid-state fermentation Potato dextrose agar
1 Introduction The world’s present economic system is highly reliant on diverse fossil energy sources such as
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