Changes in Nitric Oxide and Copper Content in Rat Liver and Hippocampus after Brain Ischemia Modeling
- PDF / 595,155 Bytes
- 6 Pages / 612 x 792 pts (letter) Page_size
- 96 Downloads / 182 Views
OSPECTROMETRIC METHODS IN SCIENCES OF LIFE
Changes in Nitric Oxide and Copper Content in Rat Liver and Hippocampus after Brain Ischemia Modeling Kh. L. Gainutdinova,b,*, V. V. Andrianova,b, G. G. Yafarovaa,b, L. V. Bazana, T. Kh. Bogodvidb, S. G. Pashkevich c, M. O. Dosinac, A. S. Zamaroc, A. A. Denisovc, and V. A. Kulchitskyc a Zavoisky
Kazan Physical Technical Institute, Kazan, Russia b Kazan University, Kazan, Russia c Institute of Physiology, National Academy of Sciences of Belarus, Minsk, Belarus *е-mail: [email protected] Received December 28, 2019; revised December 28, 2019; accepted February 17, 2020
Abstract—Results of analysis of nitric oxide and copper content in rat liver and hippocampus after brain ischemia modeling are provided. The studies are carried out using the electron paramagnetic resonance spectroscopy method with spin traps. It was shown that, the day after brain ischemia modeling, nitric oxide content in hippocampus decreases on average by 50% and a tendency toward its decrease was observed in liver tissues. Two days after brain ischemia modeling, nitric oxide content in the brain recovered and a significant increase by 46% against control indices was observed in the liver. On the second day of the postischemic period, the copper content, which is associated with superoxide dismutase content, increased on average by 2.5-fold in the liver. No significant changes in copper content was found in the hippocampus. DOI: 10.1134/S1063784220090182
INTRODUCTION The function of tissues in an organism depends on a number of factors. One such factor is the necessity for a sufficient amount of oxygen, which is delivered with the bloodstream, for oxidation processes to take place. Oxygen insufficiency results in pathological processes in the organism, which are preceded by hypoxia [1, 2]. Hypoxia is a pathological process occurring at an insufficient oxygen supply of organism tissues or impaired oxygen utilization in the course of biooxidation—a condition of oxygen starvation both of the whole organism and individual organs and tissues [2, 3]. This is an important component of pathogenesis of many diseases [4–6]. Hypoxia is a universal pathological condition in the most diverse human diseases: cardiovascular and respiratory failures, myocardial ischemia, impaired brain and peripheral circulation, etc. [2, 5, 7]. When there is an insufficient oxygen supply to an organism, and to the brain in particular, brain ischemia occurs, which may end in ischemic stroke [3, 8]. During hypoxia, the functioning of neuromediatory systems is impaired, including that of the nitric oxide (NO) system [9]. NO is known as one of the most important signal molecules, regulating the physiological function of the organism and cell metabolism. It is widespread in the nervous system [10, 11]. The participation of NO in the development of different pathological conditions in the organism is attract-
ing great interest [10, 12–14]. At present, the development of brain ischemia and the ensuing occurrence of stroke are associated
Data Loading...
