Sequestering miR165/166 enhances seed germination in Arabidopsis thaliana under normal condition and ABA treatment
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Sequestering miR165/166 enhances seed germination in Arabidopsis thaliana under normal condition and ABA treatment Shabari Sarkar Das1,2 • Manoj Majee2 • Asis K. Nandi1 • Prakash Karmakar1 Received: 11 August 2020 / Accepted: 9 September 2020 Ó Society for Plant Biochemistry and Biotechnology 2020
Abstract Seed germination is a dynamic process involving imbibition, increased metabolic activity and protrusion of a tiny plantlet rupturing the seed coat. Many genes, phytohormones like ABA and GA have been implicated in germination of Arabidopsis thaliana seeds. Although many microRNAs (miRNAs) have been shown to be differentially expressed during seed germination process, their role remains mostly unaddressed. Here we address the role of developmentally important miR165/166 in the process of seed germination. We demonstrate that the seeds of transgenic A. thaliana having target mimic-miR165/166 (eTM-miR165/166), where miR165/166 is sponged, show better germination efficiency. The seeds of this line also maintain better germination even under ABA treatment, which is a negative regulator of seed germination. Thus, our results suggest that, sequestering miR165/166 activity enhances seed germination efficiency under normal and ABA-stress condition. Keywords Seed germination miR165/166 Small RNA miRNA Stress response ABA Arabidopsis Abbreviations ABA Abscisic acid GA Gibberellic Acid miRNA MicroRNA eTM Endogenous Target Mimic HD-ZIPIII Class III HOMEODOMAIN LEUCINEZIPPER PHB PHABULOSA PHV PHAVOLUTA REV REVOLUTA ATHB8/ ARABIDOPSIS THALIANA HOMEOBOX 15 8/15 STTM Short Tandem Target Mimic & Shabari Sarkar Das [email protected] Manoj Majee [email protected] Asis K. Nandi [email protected] Prakash Karmakar [email protected] 1
Department of Botany & Forestry, Vidyasagar University, Midnapore, West Bengal, India
2
National Institute of Plant Genome and Research, Aruna Asaf Ali Marg, New Delhi 110067, India
Seed germination is a complex dynamic tri-phasic process, which involves imbibition, increased metabolic activity, and finally protrusion of the miniature plantlet by rupturing the seed coat (Bewley 1997; Bradford 2008). Research have identified many genes to be dynamically expressed and function during seed germination (Majee, et al. 2018; Oh, et al. 2004; Saxena, et al. 2013). The phytohormones Abscisic Acid (ABA) and Gibberellic Acid (GA) act antagonistically and inhibit or promote seed germination (Vishal and Kumar 2018), respectively. Research from our (Sarkar Das, et al. 2018) and other group (Liu, et al. 2007; Reyes and Chua 2007; Wang, et al. 2011) have identified many microRNAs (miRNAs) to be differentially expressed during the process of seed germination. miRNAs are small non-coding RNAs of 19–24 nucleotide length, which function by negatively regulating target genes at posttranscriptional level and have been reported to regulate various aspects of plant development (Singh, et al. 2018). In Arabidopsis, miR165/166 have been shown to regulate the de
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