Characterization of multidrug resistance in Vibrio species isolated from marine invertebrates from Andaman Sea
- PDF / 1,748,495 Bytes
- 12 Pages / 595.276 x 790.866 pts Page_size
- 72 Downloads / 179 Views
ORIGINAL ARTICLE
Characterization of multidrug resistance in Vibrio species isolated from marine invertebrates from Andaman Sea Prabla Kumari1,3 · Abhijit Poddar2,3 · Subrata K. Das3 Received: 12 May 2020 / Accepted: 18 September 2020 © King Abdulaziz City for Science and Technology 2020
Abstract This study describes the abundance of multidrug-resistant Vibrios associated with marine invertebrate hosts from the Andaman Sea, India. Thirty-eight Vibrio strains were isolated from surface mucus layers of coral Porites, Goniastrea, Pocillopora, Fungia, and eggs of spiny lobster (Panulirus penicillatus). Phenotypically, the majority of strains exhibited growth at a wide range of temperatures, salt tolerance, and diverse nutritional requirements. All the strains had more than 97% 16S rRNA sequence similarity with type species of the genus Vibrio where Vibrio fortis, and Vibrio alginolyticus were predominant. Multilocus Sequence Analysis (MLSA) using eight housekeeping genes namely ftsZ, gapA, gyrB, mreB, pyrH, recA, rpoA, and topA distributed the strains into 6 reported clades i.e., Harveyi, Ponticus, Nereis, Orientalis, Splendidus, and Mediterranei where nearly half of the total strains represented the clade Harveyi, followed by the clade Splendidus. Likewise, the PFGE profile indicated genomic heterogeneity among the strains resulting in their distribution in five major clusters. Resistance to different antimicrobials was tested following the disc diffusion method where all strains were found susceptible to chloramphenicol (30 µg) and resistant to streptomycin (10 µg), vancomycin (30 µg), sulfamethoxazole-trimethoprim (25 µg). Moreover, the resistant phenotype to other antimicrobials confirmed the abundance of multidrug resistance strains in this marine environment. Keywords Marine invertebrate · Vibrio · Multilocus sequence analysis · Genetic diversity · Drug-resistant
Introduction Vibrios are ubiquitous in the marine environment across the globe (Thompson et al. 2004; González-Escalona et al. 2008). Rapid growth with a short generation time, salt tolerance, diverse nutritional requirement, and biofilmforming capacities have acquired the adaptive abilities and Electronic supplementary material The online version of this article (https://doi.org/10.1007/s13205-020-02445-5) contains supplementary material, which is available to authorized users. * Subrata K. Das [email protected]; [email protected] 1
Present Address: CSIR‑National Chemical Laboratory, Division of Biochemical Sciences, Dr. Homi Bhabha Road, Pune 411008, India
2
Present Address: Sri Balaji Vidyapeeth University, Pillayarkuppam, National Highway 45A, Puducherry 607402, India
3
Institute of Life Sciences, Department of Biotechnology, Nalco Square, Bhubaneswar 751023, India
physiological flexibilities in the genus Vibrio to survive and flourish in the diverse oligotrophic marine environment (Takemura et al. 2014; Zhang et al. 2018). The marine Vibrios initially gained attention due to its pathogenic potentials (Stewart et al. 200
Data Loading...
