Complete Genome of Vibrio neocaledonicus CGJ02-2, An active Compounds Producing Bacterium Isolated from South China Sea
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Complete Genome of Vibrio neocaledonicus CGJ02‑2, An active Compounds Producing Bacterium Isolated from South China Sea Lin Tan1 · Isabel Gómez‑Betancur1,2 · Suxia Guo1 · Yu Ge1 · Jianping Zhao3 · Chang Chen4 · Nan Wang1 Received: 4 January 2020 / Accepted: 20 May 2020 © Springer Science+Business Media, LLC, part of Springer Nature 2020
Abstract Strain CGJ02-2 was isolated from the coral reefs in South China sea and deposited in South China Sea Institute of Oceanology, Chinese Academy of Sciences. Active compounds including indole, ρ-hydroxybenzaldehyde were isolated from this strain. To explore the biosynthetic way of these compounds and search gene clusters, the complete genome of this strain was sequenced by Single Molecule, Real-Time (SMRT) technology. It was de novo assembled to two circular chromosomes of 3,400,283 bp with GC% 44.77 and 1,845,572 bp with GC% 44.59 respectively and classified as Vibrio alginolyticus. In silico phenotype features of Vibrio alginolyticus CGJ02-2 were also analyzed. The biosynthetic pathway of ρ-hydroxybenzaldehyde and indole in this strain were postulated. Gene clusters of four secondary metabolites including bacteriocin, ectoine, siderophore, arylpolyene were identified. This study provides helpful information for further utilizing Vibrio alginolyticus CGJ02-2 as a source of valuable bioactive compounds.
Introduction ρ-hydroxybenzaldehyde is naturally found in Gastrodia elata Blume which is used in certain traditional Chinese medicines primarily aimed at ’calming the liver’ and treating headaches, dizziness, tetanus, and epilepsy [1]. Recent research Electronic supplementary material The online version of this article (https://doi.org/10.1007/s00284-020-02047-7) contains supplementary material, which is available to authorized users. * Lin Tan [email protected] * Chang Chen [email protected] 1
Haikou Experimental Station, Chinese Academy of Tropical Agricultural Sciences (CATAS), Haikou 571101, Hainan, China
2
Programa de Ofidismo/Escorpionismo, Facultad de Ciencias Farmacéuticas Y Alimentarias, Universidad de Antioquia, 1226 Medellín, Colombia
3
School of Pharmacy, National Center for Natural Products Research, Thad Cochran Research Center, University of Mississippi, University, MS 38677, USA
4
Key Laboratory of Tropical Marine Bio‑Resources and Ecology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou, Guangdong, China
has found it is involved in a host of various “biochemical activities” such as exerting significant beneficial effect on insulin resistance [2], reducing H 2O2-induced oxidative damage in microglia BV-2 cells [3] and contributing to the antiepileptic and anticonvulsive activity of Gastrodia elata B1 [4]. Therefore, it can be potentially applied to treating diabetes-type 2, neuron damage and epilepsy. In addition, it is an important intermediate used for producing medicine such as anti-arrhythmic agent (Esmolol), antibiotics (Amoxicillin), spice (Vanillin), pesticide (Dichlobenil, Bromoxynil) as we
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