Anti-quorum sensing activity of some marine bacteria isolated from different marine resources in Egypt
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ORIGINAL RESEARCH PAPER
Anti-quorum sensing activity of some marine bacteria isolated from different marine resources in Egypt Najat El-Kurdi
. Hesham Abdulla . Amro Hanora
Received: 12 December 2019 / Accepted: 1 October 2020 © Springer Nature B.V. 2020
Abstract Objectives To screen for a variety of marine bacteria with anti-quorum sensing and anti-biofilm activities. Results Among 188 bacterial isolates from water, sediment, and corals in the Red Sea region, approximately 35% (65 isolates) of the isolates displayed a significant degradation in the purple pigment of the bioreporter strain without affecting cell growth. The quorum quenching bacteria obtained from coralassociated bacteria were 66.2% out of the total isolates. The PCR amplification results revealed that the recorded Acyl Homoserine lactone (AHL) inhibition by 91% of the anti-QS marine bacteria was not due to lactonase activity. On the other hand, lactonase genes were recorded only in the remaining 9% (6 isolates) and those were belonging to genus Bacillus, Nocardiopsis, and Enterobacter based on 16S rRNA gene sequences. The results also showed that marine N. El-Kurdi (&) Department of Aquaculture Biotechnology, Fish Farming and Technology Institute, Suez Canal University, Ismailia, Egypt e-mail: [email protected] H. Abdulla Department of Botany, Faculty of Science, Suez Canal University, Ismailia, Egypt A. Hanora Department of Microbiology & Immunology, Faculty of Pharmacy, Suez Canal University, Ismailia, Egypt
bacteria with anti-QS activity inhibited 67% of the biofilm formed by Aeromonas hydrophila, Pseudomonas aeruginosa, and Vibrio alginolyticus. The computational profiling analysis confirmed the presence of the functional region in the detected genes. Conclusion Coral microbial communities are rich sources for pharmacologically important natural products with anti-quorum sensing and anti-biofilm activities. Keywords Anti-biofilm · Coral-associated bacteria · Lactonase · Quorum quenching bacteria · Red sea
Introduction Antimicrobial resistance is one of the most notable causes of morbidities and mortalities worldwide (French 2005; Dadgostar 2019; Abushaheen et al. 2020). Because of the chaotic use of antibiotics in the agricultural, veterinary, and medical sectors, many antimicrobial agents lost their efficiency. Moreover, bacteria developed resistance to different classes of antibiotics in the market (Davies and Davies 2010). By 2050, about 10 million deaths and an economic loss of US$ 100–210 trillion may occur annually as the result of such resistance, and the lowincome countries are the most affected (Dadgostar 2019). Hence, scientists work on designing new strategies to tackle this problem which caused a
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heavy economic burden (Abushaheen et al. 2020; Pietsch et al. 2020). Biofilm formation is a mechanism constructed by bacteria as a protective strategy not only from the environmental stress such as pH fluctuation and osmoregulation but also the antibio
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